Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.

<p>Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a signif...

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Príomhchruthaitheoir:	Jianhua Liao (1393228) (author)
Rannpháirtithe:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Foilsithe / Cruthaithe:	2025
Ábhair:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
Clibeanna:	Cuir clib leis Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!

Míreanna comhchosúla

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
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Foilsithe / Cruthaithe: (2025)

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Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Foilsithe / Cruthaithe: (2025)

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Foilsithe / Cruthaithe: (2025)

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
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Surface motility of the <i>yqhG</i> mutant.
de réir: Jianhua Liao (1393228)
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Membrane integrity in the <i>yqhG</i> mutant.
de réir: Jianhua Liao (1393228)
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Oxidative stress resistance of the <i>yqhG</i> mutant.
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