Alternative Ion-Pairing Modifiers Should Be Investigated in Low-Input and Single-Cell Proteomics
A recent study demonstrated a substantial increase in the peptide signal and corresponding proteome coverage when employing 0.5% acetic acid (AA) as the ion pairing modifier in place of the 0.1% formic acid traditionally used in shotgun proteomics. Given the strictly limited material and counterintu...
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2025
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