Characterization of the inhibitory activity of HR2 peptides in pseudovirus infection and cell-cell fusion.

<p>(A) Inhibition of pseudovirus infectivity by the 36-HR2 (black circles), 42-HR2 (green circles) and 36-HR2-HA (pink circles) peptides against D614G pseudovirus infection of 293T cells expressing ACE2/TMPRSS2. (B) Interaction of 42-HR1, HA-HR1 (YPYDVPDYAGPGGSGSG-42-HR1) or HR1-HA (42-HR1-GSG...

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محفوظ في:

التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Sabrina Lusvarghi (2329267) (author)
مؤلفون آخرون:	Russell Vassell (218358) (author), Brittany Williams (15236740) (author), Haseebullah Baha (17364846) (author), Sabari Nath Neerukonda (10284926) (author), Carol D. Weiss (7377635) (author)
منشور في:	2025
الموضوعات:	Biophysics Biochemistry Cell Biology Neuroscience Cancer Infectious Diseases Virology Chemical Sciences not elsewhere classified induce conformational changes expose conserved sites heptad repeat 2 trapping fusion intermediates mediate virus fusion inhibit membrane fusion findings elucidate fusion allow s1 shedding s2 transmembrane subunit s2 &# 8217 site </ p transient spike conformations spike s1 subunit 2 spike variants fusion intermediate though hr2 peptide disrupts ace2 binding alone fusion conformations 2 spikes omicron variants investigate fusion fusion conformation peptide captures intermediate conformations hr2 peptide natural s1 unwinding s2 s2 intermediate spikes lacking results indicate involve exposure intermediate relative intermediate conformation hr2 regions helix bundle electron microscopy also present ace2 engagement
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الوصف
الملخص:	<p>(A) Inhibition of pseudovirus infectivity by the 36-HR2 (black circles), 42-HR2 (green circles) and 36-HR2-HA (pink circles) peptides against D614G pseudovirus infection of 293T cells expressing ACE2/TMPRSS2. (B) Interaction of 42-HR1, HA-HR1 (YPYDVPDYAGPGGSGSG-42-HR1) or HR1-HA (42-HR1-GSGSGYPYDVPDYAGPG) and 36-HR2 peptides. Bands corresponding to 36-HR2 and the HR1/HR2 complexes are indicated. (E) Western blot of control target cells without receptor, ACE2 or ACE2 and TMPRSS2 (D) Inhibition activity of D614G spike mediated cell fusion with 293T cells expressing ACE2 (filled circles) or ACE2/TMPRSS2 (open circles) by 36-HR2.</p> <p>(TIF)</p>

Characterization of the inhibitory activity of HR2 peptides in pseudovirus infection and cell-cell fusion.

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