Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice.
<p><b>A)</b> Schematic of setup. Mice are head-fixed on a cylindrical Styrofoam treadmill and static gratings are presented on a monitor to the right eye (contralateral to photostimulated side). A spout located in front of the mouse delivers milk during rewarded trials. <b>B)...
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2025
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| 總結: | <p><b>A)</b> Schematic of setup. Mice are head-fixed on a cylindrical Styrofoam treadmill and static gratings are presented on a monitor to the right eye (contralateral to photostimulated side). A spout located in front of the mouse delivers milk during rewarded trials. <b>B)</b> Schematic of visual discrimination task. Static grating (2 s) is presented following a variable gray background inter-trial interval (ITI) lasting (3–6 s). For ‘go’ trials, the reward is delivered when licks are detected during the response window (1–2 s after stimulus onset). Depending on the presence or absence of licks, ‘go’ trials are classified as hit or miss and ‘no-go’ trials as false alarms and correct rejections, respectively. <b>C)</b> Pre- and post-learning behavioral discrimination performance, <i>d’</i>, averaged across mice (Wilcoxon signed-rank test, <i>p</i> = 0.002, <i>N</i> = 10). Gray lines represent individual mice. <b>D)</b> Schematic of optogenetic method. Optic fiber is centered over V1 to deliver blue light (473 nm) to activate Channelrhodopsin-2 (ChR2) expressed in all parvalbumin-positive interneurons of transgenic mice (PV-Cre::Ai32). <b>E)</b> Schematic of stimulation protocol. Optogenetic stimulation is delivered relative to stimulus onset (STIM ON) and offset (STIM OFF). ‘Entire’ condition (black) lasts during the entire stimulus presentation; ‘early’ condition (red) lasts from stimulus onset to 120 ms; ‘late1’ condition (blue) lasts from 120 ms to stimulus offset; ‘late2’ condition (green) lasts from 180 ms to stimulus offset. Each condition was tested in separate sessions (interleaving different power levels). <b>F)</b> Discrimination performance, <i>d’</i>, in wild-type mice (WT, <i>N</i> = 6, 7 sessions) and transgenic mice (PV-ChR2, <i>N</i> = 10, 10 sessions) at no laser stimulation (0 mW/mm<sup>2</sup>) and silencing laser power (2 mW/mm<sup>2</sup>). Circles, individual sessions; line, mean. See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003518#pbio.3003518.s001" target="_blank">S1 Fig</a>.</p> |
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