Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice.
<p><b>A)</b> Schematic of setup. Mice are head-fixed on a cylindrical Styrofoam treadmill and static gratings are presented on a monitor to the right eye (contralateral to photostimulated side). A spout located in front of the mouse delivers milk during rewarded trials. <b>B)...
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2025
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| author | Lilia Kukovska (22683586) |
| author2 | Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| author2_role | author author author author |
| author_facet | Lilia Kukovska (22683586) Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| author_role | author |
| dc.creator.none.fl_str_mv | Lilia Kukovska (22683586) Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| dc.date.none.fl_str_mv | 2025-11-25T18:37:24Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003518.g001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Learning_of_a_visual_discrimination_task_and_optogenetic_manipulations_in_head-fixed_mice_/30714559 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |
| dc.title.none.fl_str_mv | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>A)</b> Schematic of setup. Mice are head-fixed on a cylindrical Styrofoam treadmill and static gratings are presented on a monitor to the right eye (contralateral to photostimulated side). A spout located in front of the mouse delivers milk during rewarded trials. <b>B)</b> Schematic of visual discrimination task. Static grating (2 s) is presented following a variable gray background inter-trial interval (ITI) lasting (3–6 s). For ‘go’ trials, the reward is delivered when licks are detected during the response window (1–2 s after stimulus onset). Depending on the presence or absence of licks, ‘go’ trials are classified as hit or miss and ‘no-go’ trials as false alarms and correct rejections, respectively. <b>C)</b> Pre- and post-learning behavioral discrimination performance, <i>d’</i>, averaged across mice (Wilcoxon signed-rank test, <i>p</i> = 0.002, <i>N</i> = 10). Gray lines represent individual mice. <b>D)</b> Schematic of optogenetic method. Optic fiber is centered over V1 to deliver blue light (473 nm) to activate Channelrhodopsin-2 (ChR2) expressed in all parvalbumin-positive interneurons of transgenic mice (PV-Cre::Ai32). <b>E)</b> Schematic of stimulation protocol. Optogenetic stimulation is delivered relative to stimulus onset (STIM ON) and offset (STIM OFF). ‘Entire’ condition (black) lasts during the entire stimulus presentation; ‘early’ condition (red) lasts from stimulus onset to 120 ms; ‘late1’ condition (blue) lasts from 120 ms to stimulus offset; ‘late2’ condition (green) lasts from 180 ms to stimulus offset. Each condition was tested in separate sessions (interleaving different power levels). <b>F)</b> Discrimination performance, <i>d’</i>, in wild-type mice (WT, <i>N</i> = 6, 7 sessions) and transgenic mice (PV-ChR2, <i>N</i> = 10, 10 sessions) at no laser stimulation (0 mW/mm<sup>2</sup>) and silencing laser power (2 mW/mm<sup>2</sup>). Circles, individual sessions; line, mean. See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003518#pbio.3003518.s001" target="_blank">S1 Fig</a>.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_81b5ec8d71049f7fe7a26aeea8396a09 |
| identifier_str_mv | 10.1371/journal.pbio.3003518.g001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30714559 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice.Lilia Kukovska (22683586)Katharina A. Wilmes (22683589)Natsumi Y. Homma (22683592)Claudia Clopath (324706)Jasper Poort (22683595)BiochemistryNeuroscienceDevelopmental BiologyScience PolicyMental HealthBiological Sciences not elsewhere classifiedxlink "> inhibitionthree major factorsstrong competitive interactionsphoton imaging showedbalanced cortical activityprimary visual cortexsustained v1 activitycombined optogenetic activationv1 cells capturedmoderate pv activationmoderate activationvisual discriminationsexpressing cellsnonlinear activationdependent activationactivation strengthtask difficultystimulus onsetperceptual discriminationdifficult discriminationsdelineates conditionscircuit modelbehavioral effects<p><b>A)</b> Schematic of setup. Mice are head-fixed on a cylindrical Styrofoam treadmill and static gratings are presented on a monitor to the right eye (contralateral to photostimulated side). A spout located in front of the mouse delivers milk during rewarded trials. <b>B)</b> Schematic of visual discrimination task. Static grating (2 s) is presented following a variable gray background inter-trial interval (ITI) lasting (3–6 s). For ‘go’ trials, the reward is delivered when licks are detected during the response window (1–2 s after stimulus onset). Depending on the presence or absence of licks, ‘go’ trials are classified as hit or miss and ‘no-go’ trials as false alarms and correct rejections, respectively. <b>C)</b> Pre- and post-learning behavioral discrimination performance, <i>d’</i>, averaged across mice (Wilcoxon signed-rank test, <i>p</i> = 0.002, <i>N</i> = 10). Gray lines represent individual mice. <b>D)</b> Schematic of optogenetic method. Optic fiber is centered over V1 to deliver blue light (473 nm) to activate Channelrhodopsin-2 (ChR2) expressed in all parvalbumin-positive interneurons of transgenic mice (PV-Cre::Ai32). <b>E)</b> Schematic of stimulation protocol. Optogenetic stimulation is delivered relative to stimulus onset (STIM ON) and offset (STIM OFF). ‘Entire’ condition (black) lasts during the entire stimulus presentation; ‘early’ condition (red) lasts from stimulus onset to 120 ms; ‘late1’ condition (blue) lasts from 120 ms to stimulus offset; ‘late2’ condition (green) lasts from 180 ms to stimulus offset. Each condition was tested in separate sessions (interleaving different power levels). <b>F)</b> Discrimination performance, <i>d’</i>, in wild-type mice (WT, <i>N</i> = 6, 7 sessions) and transgenic mice (PV-ChR2, <i>N</i> = 10, 10 sessions) at no laser stimulation (0 mW/mm<sup>2</sup>) and silencing laser power (2 mW/mm<sup>2</sup>). Circles, individual sessions; line, mean. See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003518#pbio.3003518.s001" target="_blank">S1 Fig</a>.</p>2025-11-25T18:37:24ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003518.g001https://figshare.com/articles/figure/Learning_of_a_visual_discrimination_task_and_optogenetic_manipulations_in_head-fixed_mice_/30714559CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307145592025-11-25T18:37:24Z |
| spellingShingle | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. Lilia Kukovska (22683586) Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |
| status_str | publishedVersion |
| title | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| title_full | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| title_fullStr | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| title_full_unstemmed | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| title_short | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| title_sort | Learning of a visual discrimination task and optogenetic manipulations in head-fixed mice. |
| topic | Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |