PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production in <i>Kluyveromyces marxianus</i>

PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production in <i>Kluyveromyces marxianus</i>

Monoterpenes are valued for their roles as flavors, fragrances, insecticides, and energy-dense fuels. Microorganisms provide sustainable biosynthesis routes for these important molecules, but production levels remain limited. Here, we introduce a biosensor-driven microbial engineering strategy to en...

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Bibliographic Details
Main Author: Nicholas R. Robertson (21699212) (author)
Other Authors: Chase Lenert-Mondou (21699215) (author), Alison C. Leonard (21699218) (author), Aida Tafrishi (21699221) (author), Stephanie Carrera (21699224) (author), Sangcheon Lee (21699227) (author), Yuna Aguilar (21699230) (author), Leonardo Sanchez Zamora (21699233) (author), Trinity Nguyen (21699236) (author), Jesús Beltrán (17026608) (author), Mengwan Li (9951863) (author), Sean R. Cutler (4521637) (author), Timothy A. Whitehead (640441) (author), Ian Wheeldon (610257) (author)
Published: 2025
Subjects:
Biochemistry
Medicine
Microbiology
Genetics
Biotechnology
Plant Biology
Space Science
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Information Systems not elsewhere classified
using mutagenized libraries
rapid strain engineering
kluyveromyces marxianus </
improved monoterpene production
enhance monoterpene production
cas9 mutagenesis approach
sensitive pyr1 variant
sensitive pyr1 sensor
pyr1 receptor 
specifically targeting geraniol
highly selective geraniol
enhance geraniol production
based biosensor circuit
wide crispr screens
screened 24 monoterpenes
pyr1 biosensor platform
wide crispr
pyr1 biosensor
including geraniol
versatile biosensor
thermotolerant yeast
study demonstrates
low background
important molecules
fold increase
desired metabolite
dense fuels
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