PCR analysis of additional CTΔincA mutant clones.

gDNA was isolated from WT C. trachomatis L2 and varying temporal isolates (T1-T8) of the two clones indicated and analyzed by PCR using primers located outside the upstream and downstream ~2 kb flanking sequences. Lower weight bands containing the WT &l...

وصف كامل

محفوظ في:

التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Yibing Wang (204330) (author)
مؤلفون آخرون:	Robert Suchland (16752729) (author), Amy Hua (20208626) (author), Steven Carrell (11846926) (author), Daniel Rockey (11846929) (author), Kevin Hybiske (129225) (author)
منشور في:	2024
الموضوعات:	Microbiology Genetics Ecology Science Policy Infectious Diseases Space Science Biological Sciences not elsewhere classified powerful new strategy new strategies need model chlamydial strains efficiently target loci div >< p chloramphenicol resistance marker >- infected monolayers >, including deletions host cells infected numerous gene targets whole genome sequencing deletion mutants containing making gene deletion chlamydia trachomatis </ trachomatis </ chlamydia </ replacement mutants mccoy cells gene deletion muridarum </ inca </ efficient deletion deletion remains c </ uniform presence targeting sequences resulting clones phenotypic assessment nonfusogenic vacuoles multiple genes inca expression immunofluorescence microscopy desirable goal based genotyping 2 rounds
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!

PCR analysis of additional CTΔincA mutant clones.

مواد مشابهة