Biochemical investigation of wild-type and processed RNG2.

Biochemical investigation of wild-type and processed RNG2.

<p><b>(A)</b> Representation of the RNG2 constructs expressed in SF9 insect cells. <b>(B)</b> Insect cell protein expression and solubility of the RNG2 truncation variants, as assessed by Coomassie blue staining. <b>(C)</b> Protein expression and solubility...

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מחבר ראשי:	Romuald Haase (22339777) (author)
מחברים אחרים:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
יצא לאור:	2025
נושאים:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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פריטים דומים

Investigation of RNG2 subdomain functions.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Multiple epitopes-tagging across RNG2.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

RNG2 is functional only as intact, full-length protein.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Additional information on the RNG2 variants expressed in UPRT locus.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Additional information on the processed forms and localization of the RNG2 protein.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Additional information of the RNG2 purification from insect cells.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Model RNG2 as a molecular tether for conoid apical anchorage.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

RNG2 spans between the APR and the conoid, forming 22 tethers.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Additional information on the cryo-electron tomography performed on RNG2-mAID strain.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Phenotypic consequences of the conoid detachment.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

<i>Toxoplasma gondii</i> tachyzoite cell organization.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Reconstruction of the Fig 4E panel III.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Reconstruction of the Fig 4E panel II.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Reconstruction of the Fig 4E panel I.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Source Numerical Data of this study.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Additional information on antibodies and primers used in this study.
מאת: Romuald Haase (22339777)
יצא לאור: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
מאת: Pengchao Zheng (22674589)
יצא לאור: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
מאת: Pengchao Zheng (22674589)
יצא לאור: (2025)

A. Mouse transcriptome non-negative matrix factorization component weights across all time points and conditions. Larger weight values indicate components with upregulated genes dominating. B. Gene set enrichment plot for selected features from each component in panel A. Components 1, 4, and 5 did not exhibit enrichment in these broad terms. C. Number of genes in enriched gene ontology terms related to immunity, signaling, and transcription for each time point and condition. D. Time course transcriptional profiles for mouse genes characteristic to component 3. E. PARFA-seq validation by quantitative PCR and gene expression changes during immortalized bone marrow-derived macrophage infection by <i>T. gondii</i> assessed by quantitative PCR.
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

A. <i>T. gondii</i> gene group changes throughout the time course, expressed as log<sub>2</sub>(fold change) relative to pre-infection tachyzoites (t = 0). Grey area depicts the 95% confidence interval. n value refers to number of genes detected in each group. B. Gene set enrichment plot for most enriched terms from <i>T. gondii</i> transcriptome features at 15 min to 120 min. Five most significant terms are displayed for each database. C and D. Transcriptional profiles of top five upregulated and downregulated <i>T. gondii</i> transcription factors, respectively.
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

A. Library preparation strategy: poly-A(+) RNAs are captured by complementarity to reverse transcription primer, then second strand is synthesized using random hexamers and handles for sequencing are attached through two rounds of PCR. B. Mapped read distribution by species and by biotype. C and D. Principal component analysis of mouse and <i>T. gondii</i> transcriptomes, respectively. E and F. Volcano plots of Live <i>Toxoplasma</i>-infected mouse and <i>T. gondii</i> time points, respectively. To calculate Log<sub>2</sub>(Fold change), every timepoint is compared to corresponding samples (either macrophage or <i>Toxoplasma</i>) before infection (t = 0 min). Most significant and most upregulated transcripts are labelled. Number of significantly (p ≤ 0.05) downregulated and upregulated genes for each time point is given in blue and red, respectively. In F, 24-hour time point, red dots mark ROP
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

A. Experimental scheme of infection time course and downstream workflow; B. Optimization of tachyzoite concentration to achieve adequate infection rates, with a threshold set at 50% infection efficiency. Error bars represent ± 1 standard deviation. Corresponding MOI values are given for each condition; C. Infection time course micrograph merged from brightfield and green channels. <i>T. gondii</i> exhibits green fluorescence due to stable GFP expression in the strain used. Percentage at the top left indicates infection efficiency (± 1 standard deviation) for each time point.
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

Summary of all significant gene enrichment terms for every condition (as combination of sample type and timepoint).
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

Oligonucleotides used in this study.
מאת: Dominykas Murza (22676289)
יצא לאור: (2025)

<b>No effect of actual parasitism risk on egg rejection in two sympatric breeding cuckoo hosts</b>
מאת: Hanlin Yan (19840763)
יצא לאור: (2025)

Transcriptomic insights into the tripartite plant–pathogen–mycoparasite interaction reveal novel mechanisms involving fungal secretomes and plant amino acid metabolism
מאת: kazuya maeda (17387962)
יצא לאור: (2025)

Summary of the general phenotype of SH3 domain-containing gene mutants.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

SH3 domain-containing genes and the subcellular localization of their respective products in <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

The IDRs in SH3 domain-containing proteins are important for the fusion of cytoplasmic protein condensates.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

SH3 domain-containing proteins exhibit features of phase separation in <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Effects of <i>FgHOF1</i> deletion on cytoskeletal and nuclear distribution.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Co-localization of FgAip5-GFP at hyphal tips and various SH3-domain proteins at membrane-associated structures.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Subcellular localizations of SH3 domain-containing proteins in <i>F. graminearum</i> hyphae.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Role of SH3 domain-containing proteins on deoxynivalenol (DON) production in <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Effect of SH3 domain gene deletions on the pathogenicity of <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Influence of SH3 domain-containing proteins on sexual development of <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Conidiation and conidial morphology of the SH3 domain-containing gene mutants.
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

Impact of SH3 domain-containing proteins on the vegetative growth of <i>F. graminearum.</i>
מאת: Yakubu Saddeeq Abubakar (5558399)
יצא לאור: (2025)

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