Biochemical investigation of wild-type and processed RNG2.

Biochemical investigation of wild-type and processed RNG2.

<p><b>(A)</b> Representation of the RNG2 constructs expressed in SF9 insect cells. <b>(B)</b> Insect cell protein expression and solubility of the RNG2 truncation variants, as assessed by Coomassie blue staining. <b>(C)</b> Protein expression and solubility...

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Bibliografiska uppgifter
Huvudupphovsman:	Romuald Haase (22339777) (author)
Övriga upphovsmän:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Publicerad:	2025
Ämnen:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Additional information on the processed forms and localization of the RNG2 protein.
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Additional information of the RNG2 purification from insect cells.
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RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
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Reconstruction of the Fig 4E panel III.
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Reconstruction of the Fig 4E panel II.
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Reconstruction of the Fig 4E panel I.
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Source Numerical Data of this study.
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