SYTOX Green fluorescence measurements for membrane permeability assessment.

SYTOX Green fluorescence measurements for membrane permeability assessment.

<p>SYTOX Green fluorescence measurements for membrane permeability assessment.</p>

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Hlavní autor:	Jianhua Liao (1393228) (author)
Další autoři:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Vydáno:	2025
Témata:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
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Podobné jednotky

Primers Used in This Study.
Autor: Jianhua Liao (1393228)
Vydáno: (2025)

Bacterial Strains and Plasmids Used in This Study.
Autor: Jianhua Liao (1393228)
Vydáno: (2025)

Oxidative stress resistance of the <i>yqhG</i> mutant.
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Vydáno: (2025)

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
Autor: Jianhua Liao (1393228)
Vydáno: (2025)

Surface motility of the <i>yqhG</i> mutant.
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Vydáno: (2025)

Membrane integrity in the <i>yqhG</i> mutant.
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Vydáno: (2025)

Biofilm formation in MRSA strains.
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Vydáno: (2025)

YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
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Vydáno: (2025)

Viable CFU counts following hydrogen peroxide exposure.
Autor: Jianhua Liao (1393228)
Vydáno: (2025)

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
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Vydáno: (2025)

Colony diameter measurements from soft agar motility assays.
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Vydáno: (2025)

Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
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Vydáno: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
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Vydáno: (2025)

Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
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Vydáno: (2025)

Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Vydáno: (2025)

Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Vydáno: (2025)

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
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Composition of intestinal microbial communities in the small intestine of rats before and after <i>T. gondii</i> infection.
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Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
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Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
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Differential metabolite results, listing metabolites with significant changes across treatment groups, including fold changes, and <i>p</i>-values.
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Orthogonal partial least squares-discriminant analysis (OPLS-DA) of serum metabolomics data.
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Quality control assessment of serum metabolomics data.
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Heatmap of enriched KEGG pathways across samples, illustrating variations in pathway abundance.
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Graphical abstract.
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The U sequence method.
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