Cell sorting strategy for the isolation of HSCs and VSELs from peripheral whole blood.

Cell sorting strategy for the isolation of HSCs and VSELs from peripheral whole blood.

<p>Immunostained cells were first visualized by dot plot showing forward scatter (FSC) vs. side scatter (SSC) signals, where small events ranging from 2–15 μm were gated (P1) (<b>A</b>) and further analyzed for the expression of Lineage markers. Lineage negative events were gated (...

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Bibliographic Details
Main Author: Justyna Jarczak (4092082) (author)
Other Authors: Kamila Bujko (538703) (author), Katarzyna Brzeźniakiewicz-Janus (20536170) (author), Mariusz Ratajczak (3556316) (author), Magdalena Kucia (15076185) (author)
Published: 2025
Subjects:
Biochemistry
Cell Biology
Genetics
Molecular Biology
Biotechnology
Developmental Biology
Cancer
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
several human tissues
like stem cells
hematopoietic stem cells
extremely small number
xlink "> populations
step ngs protocol
generation sequencing protocol
cd45 -), circulating
small numbers
small embryonic
transcriptomic profiles
step overview
sorting strategies
seq analysis
peripheral blood
library preparation
isolated populations
good quality
cell populations
cd45 +)
bulk rna
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