Quantification of cytokine and myeloperoxidase release upon EV stimulation.

Quantification of cytokine and myeloperoxidase release upon EV stimulation.

<p>Monocytes were stimulated for 24 h <i>in vitro</i> with 1000 EVs/cell or equal volume mock control. Supernatants of stimulated cells were analyzed by ELISA or flow cytometry-based multiplex cytokine assay (LEGENDplex). To control for the effect of protein denaturation on stimula...

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Main Author: Barbara Honecker (10328267) (author)
Other Authors: Valentin A. Bärreiter (17686338) (author), Katharina Höhn (7444811) (author), Balázs Horváth (438763) (author), Karel Harant (6598289) (author), Nahla Galal Metwally (10328273) (author), Claudia Marggraff (17686347) (author), Juliett Anders (17697131) (author), Stephanie Leyk (21071975) (author), Maria del Pilar Martínez-Tauler (21071978) (author), Annika Bea (17686341) (author), Charlotte Hansen (17686335) (author), Helena Fehling (3091602) (author), Melanie Lütkemeyer (21071981) (author), Stephan Lorenzen (3091605) (author), Sören Franzenburg (649055) (author), Hanna Lotter (8745888) (author), Iris Bruchhaus (32227) (author)
Published: 2025
Subjects:
Medicine
Microbiology
Physiology
Immunology
Developmental Biology
Space Science
upregulated gene expression
total protein content
tnf signaling ).
stronger immune response
signaling peptides compared
secretes extracellular vesicles
key signaling pathways
host immune system
host immune response
higher monocyte recruitment
factors potentially involved
div >< p
biased immunopathology mediated
activation marker cd38
inflammatory monocyte signaling
inflammatory immune responses
inflammatory immune response
increased surface presentation
entamoeba histolytica </
amebic liver abscesses
released evs contribute
hi </ sup
b2 ev stimulation
histolytica </
inflammatory cytokines
liver damage
eh </
b2 amebae
inflammatory monocytes
monocytes contribute
severe pathology
results suggest
providing insight
parasitic protozoan
mirna cargo
men exhibit
low pathogenic
isolated evs
including nf
highly pathogenic
far little
evs secreted
evs ),
derived cells
amebic pathogenicity
alas ),
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