IFA effectively limited bacterial dissemination and protected mice from lethal hypervirulent <i>K</i>. <i>pneumoniae</i> pneumonia.
<p>(A) Survival of mice 72 hours after <i>K</i>. <i>pneumoniae</i> infection. Mice were subcutaneously injected with 50 mg/kg IFA in 50 μl of 10% DMSO containing vehicle (10% DMSO, 45% stroke-physiological saline solution, 40% PEG400 and 5% Tween-80) or an equal volume...
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2025
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| Summary: | <p>(A) Survival of mice 72 hours after <i>K</i>. <i>pneumoniae</i> infection. Mice were subcutaneously injected with 50 mg/kg IFA in 50 μl of 10% DMSO containing vehicle (10% DMSO, 45% stroke-physiological saline solution, 40% PEG400 and 5% Tween-80) or an equal volume of vehicle immediately after challenge with 1 × 10<sup>7</sup> wild-type <i>K</i>. <i>pneumoniae</i> K7 or <i>ΔGT-1</i> K7 bacteria, and the number of deaths was recorded for survival analysis. Statistical analysis was performed using the log-rank (Mantel–Cox) test (n = 12 mice each group). (B) Bacterial burden in the lung tissues of sublethally infected mice. Mice challenged with 2.5 × 10<sup>6</sup> wild-type <i>K</i>. <i>pneumoniae</i> K7 or <i>ΔGT-1</i> K7 bacteria were treated as described above and sacrificed at 40 hours post infection, and lung tissues were removed and homogenized in sterilized PBS (10% w/v) to analyze the bacterial burden by microbiological plating (n = 5 mice each group). (C) Bacterial load in the bronchoalveolar lavage fluid (BALF) obtained from sublethally infected mice. Mice that were infected and treated as described above were sacrificed at 32 hours post infection for bronchoalveolar lavage (BAL) analysis, and the BALF was serially diluted and microbiologically plated (n = 6 mice in each group). The bacterial loads in the livers (D) and spleens (E) from the mice mentioned in (B) were also evaluated by microbiologically plating the tissue homogenates in PBS (n = 5 mice each group). ND, not detected. The back lines present the means ± SEMs. (F) H&E-stained lung tissues from mice infected and treated as indicated. The arrows indicate neutrophil infiltration. The degree of airway inflammation (G), neutrophilic infiltration (H), intralesional bacterial burden (I) and total histopathology score (J) were assessed according to standard pathology criteria by blinded scoring. The data are presented as the means ± SEMs. Unpaired two-tailed Student’s t-test was performed to determine the statistical significance of two groups. *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ns, no significance.</p> |
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