Overview of significant O. lignaria life stages and protocol steps for this study.

(A) and (B) are images of the two life stages used in this study: the second instar and the late fifth instar beginning to spin silk, respectively. The red arrow and circle indicate a cut made in the silk mesh to access the larva’s mouth. (C) A single silk strand is isola...

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Bibliographic Details
Main Author:	Oran Wasserman (20209911) (author)
Other Authors:	Jackson J. Morley (20790586) (author), Mary-Kate F. Williams (20790589) (author), Brianne E. Bell (1569412) (author), Diana L. Cox-Foster (20790592) (author), Justin A. Jones (1329945) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Medicine Genetics Biotechnology Evolutionary Biology Ecology Developmental Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified various arthropod lineages div >< p based fiber spun produces silk cocoons naturally spun silk enabling silk characterization fifth instar larvae minimally invasive protocol silk fibers using osmia lignaria </ 20 fibers isolated bee silk properties lignaria </ isolated fibers isolated silk mechanical properties bee biology silk initiation cocoon silk spinning larvae seven larvae successfully isolated protocol provides protocol allows effective protocol solitary bees reveal physical remarkable protein producing species practical tool nutritional status morphological characteristics mechanically test mechanical testing limited information investigate biology extensively studied domesticated silkworms developmental studies
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Overview of significant <i>O. lignaria</i> life stages and protocol steps for this study.

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