STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry.
<p>(B) THP-1 cells were incubated with HLA-E (VLA) coated microbeads at either 37°C or 4°C and analysed by flow cytometry. (C) THP-1 cells were incubated with fluorescent microbeads coated with peptide-loaded HLA molecules for either 1h or 24h and analysed using flow cytometry. The percentage...
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2025
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| _version_ | 1852015831477649408 |
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| author | Tom A. W. Schoufour (22421454) |
| author2 | Linda Voogd (20466968) Kees L.M.C. Franken (14983373) Tom H.M. Ottenhoff (11686365) Ruud H.M. Wijdeven (22421457) Simone A. Joosten (11074623) |
| author2_role | author author author author author |
| author_facet | Tom A. W. Schoufour (22421454) Linda Voogd (20466968) Kees L.M.C. Franken (14983373) Tom H.M. Ottenhoff (11686365) Ruud H.M. Wijdeven (22421457) Simone A. Joosten (11074623) |
| author_role | author |
| dc.creator.none.fl_str_mv | Tom A. W. Schoufour (22421454) Linda Voogd (20466968) Kees L.M.C. Franken (14983373) Tom H.M. Ottenhoff (11686365) Ruud H.M. Wijdeven (22421457) Simone A. Joosten (11074623) |
| dc.date.none.fl_str_mv | 2025-10-13T17:23:41Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0334543.g004 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/STAB1_or_STAB2_expression_increases_THP-1_phagocytotic_uptake_of_HLA-E_coated_beads_A_THP-1_dCas9_cells_were_transduced_with_a_control_guide_or_a_guide_targeting_either_LILRB1_STAB1_or_STAB2_stained_with_HLA_tetramers_and_analysed_by_flow_c/30347997 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Cell Biology Immunology Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified mycobacterium tuberculosis </ communicates cellular health cas9 activation screen low affinity peptides also present peptides identify additional hla e coated microbeads two novel hla div >< p 2 </ p peptides derived novel interactors specific hla stabilin receptors results provide natural killer leader sequence inhibit phagocytosis identified stabilin first evidence e tetramers e receptors e complex canonical role |
| dc.title.none.fl_str_mv | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(B) THP-1 cells were incubated with HLA-E (VLA) coated microbeads at either 37°C or 4°C and analysed by flow cytometry. (C) THP-1 cells were incubated with fluorescent microbeads coated with peptide-loaded HLA molecules for either 1h or 24h and analysed using flow cytometry. The percentage of cells with signal above untreated controls is plotted as mean ± SD. (D) Representative Z-stack of images gathered by confocal microscopy of a THP-1 gSTAB1 cell incubated with HLA-E (VLA) coated fluorescent beads (green). CellMask membrane staining is depicted in red. All data represent at least three independent experiments.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_a2d445a38fc06b10fa3cb568d10fa607 |
| identifier_str_mv | 10.1371/journal.pone.0334543.g004 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30347997 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry.Tom A. W. Schoufour (22421454)Linda Voogd (20466968)Kees L.M.C. Franken (14983373)Tom H.M. Ottenhoff (11686365)Ruud H.M. Wijdeven (22421457)Simone A. Joosten (11074623)BiochemistryCell BiologyImmunologyInfectious DiseasesBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedmycobacterium tuberculosis </communicates cellular healthcas9 activation screenlow affinity peptidesalso present peptidesidentify additional hlae coated microbeadstwo novel hladiv >< p2 </ ppeptides derivednovel interactorsspecific hlastabilin receptorsresults providenatural killerleader sequenceinhibit phagocytosisidentified stabilinfirst evidencee tetramerse receptorse complexcanonical role<p>(B) THP-1 cells were incubated with HLA-E (VLA) coated microbeads at either 37°C or 4°C and analysed by flow cytometry. (C) THP-1 cells were incubated with fluorescent microbeads coated with peptide-loaded HLA molecules for either 1h or 24h and analysed using flow cytometry. The percentage of cells with signal above untreated controls is plotted as mean ± SD. (D) Representative Z-stack of images gathered by confocal microscopy of a THP-1 gSTAB1 cell incubated with HLA-E (VLA) coated fluorescent beads (green). CellMask membrane staining is depicted in red. All data represent at least three independent experiments.</p>2025-10-13T17:23:41ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0334543.g004https://figshare.com/articles/figure/STAB1_or_STAB2_expression_increases_THP-1_phagocytotic_uptake_of_HLA-E_coated_beads_A_THP-1_dCas9_cells_were_transduced_with_a_control_guide_or_a_guide_targeting_either_LILRB1_STAB1_or_STAB2_stained_with_HLA_tetramers_and_analysed_by_flow_c/30347997CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/303479972025-10-13T17:23:41Z |
| spellingShingle | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. Tom A. W. Schoufour (22421454) Biochemistry Cell Biology Immunology Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified mycobacterium tuberculosis </ communicates cellular health cas9 activation screen low affinity peptides also present peptides identify additional hla e coated microbeads two novel hla div >< p 2 </ p peptides derived novel interactors specific hla stabilin receptors results provide natural killer leader sequence inhibit phagocytosis identified stabilin first evidence e tetramers e receptors e complex canonical role |
| status_str | publishedVersion |
| title | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| title_full | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| title_fullStr | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| title_full_unstemmed | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| title_short | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| title_sort | STAB1 or STAB2 expression increases THP-1 phagocytotic uptake of HLA-E coated beads (A) THP-1 dCas9 cells were transduced with a control guide or a guide targeting either LILRB1, STAB1 or STAB2 stained with HLA tetramers and analysed by flow cytometry. |
| topic | Biochemistry Cell Biology Immunology Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified mycobacterium tuberculosis </ communicates cellular health cas9 activation screen low affinity peptides also present peptides identify additional hla e coated microbeads two novel hla div >< p 2 </ p peptides derived novel interactors specific hla stabilin receptors results provide natural killer leader sequence inhibit phagocytosis identified stabilin first evidence e tetramers e receptors e complex canonical role |