Optimization of the Nb-ELISA using HRP conjugated Nanobodies.

<p>Absorbance values at 450 nm obtained from two-dimensional titration using different concentrations of Nb14 Fc as the capture antibody and Nb48 HRP (A) or Nb66 HRP (C) as the detection antibody. The assay was performed with and without 0.5 μg/mL of NS1-2. P/N ratio for combinations of Nb14 F...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	María Florencia Pavan (22538063) (author)
مؤلفون آخرون:	Cristian Miguel Malnero (22538066) (author), María Emilia Mora Alvarado (22538069) (author), María Camila Carzoglio (22538072) (author), Yesica Paredes Rojas (22538075) (author), Agostina Bruno (9708143) (author), Laura Perez Vidakovics (14372943) (author), Leo Hanke (14245409) (author), Alejandro Castello (11822201) (author), Gerald McInerney (167172) (author), Cybele Carina García (22538078) (author), Viviana Parreño (409570) (author), Lorena Itatí Ibañez (22538081) (author)
منشور في:	2025
الموضوعات:	Medicine Genetics Biotechnology Cancer Infectious Diseases Virology Computational Biology system exhibits limitations successfully identified cases sera pool dilutions public health system cases increasing sharply borne flavivirus responsible target ns1 across reported ns1 concentrations precision diagnostic tools 3 &# 8211 four denv serotypes denv genome encodes structural protein 1 6 &# 8211 early infection detection ns1 ), diagnostic sensitivity detecting ns1 3 %). denv ), xlink "> urgent need symptom onset sporadic disease results underscore regions facing recurrent epidemic recent decades limited access key biomarker human samples exposing gaps engineered nanobodies detection antibodies detection aligning detected signals affecting 18 accurate diagnosis 9 ), 6 %) 23 provinces
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Optimization of the Nb-ELISA using HRP conjugated Nanobodies.

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