Candidate CIA pathway proteins are important for parasite proliferation.
<p><b>(A–G)</b> Fluorescence proliferation assays and western blotting of <b>(A)</b> r<i>Tg</i>Tah18-mAID-HA, <b>(B)</b> r<i>Tg</i>Dre2-mAID-HA, <b>(C)</b> rHA-mAID-<i>Tg</i>Nar1, <b>(D)</b> r<i&...
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2025
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| Čoahkkáigeassu: | <p><b>(A–G)</b> Fluorescence proliferation assays and western blotting of <b>(A)</b> r<i>Tg</i>Tah18-mAID-HA, <b>(B)</b> r<i>Tg</i>Dre2-mAID-HA, <b>(C)</b> rHA-mAID-<i>Tg</i>Nar1, <b>(D)</b> r<i>Tg</i>CIA1-mAID-HA, <b>(E)</b> r<i>Tg</i>CIA2-mAID-HA, <b>(F)</b> r<i>Tg</i>MMS19-mAID-HA, and <b>(G)</b> WT (RH<i>∆ku80</i>/Tir1-FLAG/tdTomato) parasites cultured in the absence (black) or presence (red) of IAA. Parasite proliferation is expressed as a percentage of the final fluorescence measurement in the -IAA condition for each line. Individual data points and error bars represent the mean ± SD of three technical replicates. Data are representative of three independent experiments. Error bars not visible are smaller than the symbol. The numerical data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003520#pbio.3003520.s016" target="_blank">S1 Data</a>. For the western blotting, parasites were cultured in the absence (−) or presence (+) of IAA for 24 h, with protein samples separated by SDS-PAGE then subjected to western blotting with anti-HA antibodies to detect the mAID-HA-tagged protein of interest and anti-<i>Tg</i>Tom40 antibodies as a loading control (<b>A–F</b>, left).</p> |
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