Workflow of casp-6 sample preparation and optimization for NMR spectroscopy.

Workflow of casp-6 sample preparation and optimization for NMR spectroscopy.

<p>Each step in the protein production, purification, and exchange process was explored and assessed by multiple tests. Key areas of manipulation are noted.</p>

Furkejuvvon:

Bibliográfalaš dieđut
Váldodahkki:	Nathanael J. Kuzio (6298328) (author)
Eará dahkkit:	Marco Tonelli (178598) (author), Jasna Fejzo (2306863) (author), Jeanne A. Hardy (1514026) (author)
Almmustuhtton:	2025
Fáttát:	Biophysics Biochemistry Cell Biology Biotechnology Cancer Computational Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified potential inhibition mechanisms parkinson &# 8217 insufficient protein concentration human neurodegenerative diseases alzheimer &# 8217 difficult protein samples dimensional biomolecular nmr heteronuclear nmr studies destructive protease caspase 6 </ p previous studies difficult family yields suitable ultimately led study caspase structural transition sample stability resolution insight requires atomic ray crystallography prior attempts obtain isotopically labeled caspase homogeneity due failed due exploratory strategy enabled us conformational transitions assist others apoptotic family 6 undergoes
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