Nitrocefin and p-nitrophenyl butyrate hydrolysis assays for Pseudomonas <i>aeruginosa</i> TesA.

Nitrocefin and p-nitrophenyl butyrate hydrolysis assays for Pseudomonas <i>aeruginosa</i> TesA.

<p><b>(A)</b> Purified <i>P. aeruginosa</i> TesA with N-terminal tag (PaTesA). <b>(B)</b> Representative example of nitrocefin hydrolysis by PaTesA. Red nested curves represent a series of spectral measurements taken over time. Blue curves indicate the contr...

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Bibliografiske detaljer
Hovedforfatter:	Martin B. L. McAndrew (22683641) (author)
Andre forfattere:	Jonathan Cook (3435386) (author), Amy Gill (14910614) (author), Kavya Sahoo (22683644) (author), Clare Thomas (7943747) (author), Phillip J. Stansfeld (1267617) (author), Allister Crow (263807) (author)
Udgivet:	2025
Fag:	Biophysics Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Information Systems not elsewhere classified tolc efflux pump multifunctional hydrolytic enzyme extracts hydrophobic compounds extracting hydrophobic molecules capture implied long bacterial cell envelope active site residues lipid hydrolase complex escherichia coli </ data suggests ybbap abc superfamily ’ div >< p coli </ abc superfamily thioester substrates tesa complex structural characterization remarkable diversity new function mechanotransmission mechanism inner membrane extensively characterized experimentally confirm complete ybbap >, three
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Lignende værker

p-Nitrophenyl butyrate hydrolysis data underpinning Fig 4A in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Nitrocefin hydrolysis data underpinning Fig 4D in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Amino acid sequence conservation in TesA.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Esterase and thioesterase activity of YbbAP-TesA.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Identification of TesA as the periplasmic partner of YbbAP.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Evidence for a mechanotransmission mechanism in YbbAP-TesA.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Structures of YbbAP and the YbbAP-TesA complex.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Evidence for a mechanotransmission mechanism in YbbAP-TesA.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Comparison of YbbAP-TesA and BceAB-BceS.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Mutational analysis of the TesA active site and in vivo esterase activity.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Coevolving residue pairs mapped to the YbbP-TesA interface.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Roving camera tour of the cryoEM map underpinning the YbbAP-TesA structure.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Image processing workflow for the AMP-PNP-bound YbbAP-TesA complex cryo-EM reconstruction.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Ester hydrolysis data underpinning Fig 4E in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Comparative rates of hydrolysis underpinning Fig 4B in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

In vivo ester hydrolysis data underpinning Fig 5A in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Acetyl CoA hydrolysis data underpinning Fig 4C in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Biological functions of the four Type VII ABC transporters in <i>Escherichia coli.</i>
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

<i>In silico</i> pull-down data underpinning Fig 1A in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Molecular dynamics simulations of YbbAP suggest the periplasmic-side pocket is a lipid-binding site.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Contact propensity data underpinning S9 Fig in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Molecular dynamics data underpinning S8 Fig in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Enzymatic data underpinning S5C Fig in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Enzymatic data underpinning S5B Fig in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Raw SDS-PAGE gel underpinning S5A Fig in PowerPoint format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Molecular dynamics data underpinning Fig 6C in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Raw SDS-PAGE gels underpinning Fig 1D in PowerPoint format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Coevolution data underpinning histogram in Fig 1C in Excel format.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Atomistic molecular dynamics simulation showing partial extraction of a lipid from the bilayer by YbbAP.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

LysoPG trajectory in atomistic simulation of YbbAP.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Root-mean-square fluctuation (RMSF) and Root-mean-square deviation (RMSD) calculations for atomistic simulations of YbbAP.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Lipid residency distributions during coarse-grained simulations of YbbAP.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Image processing workflow for the apo YbbAP cryo-EM reconstruction.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Image processing workflow for the AMP-PNP-bound YbbAP cryo-EM reconstruction.
af: Martin B. L. McAndrew (22683641)
Udgivet: (2025)

Drug-bound Outward-facing Conformation of a Heterodimeric ABC Exporter Suggests a Putative Mechanism of Drug Translocation
af: Qingyu Tang, (20785488)
Udgivet: (2025)

Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx
af: Pfariso Maumela (18462231)
Udgivet: (2025)

Additional file 2 of Characterization of a novel Jumbo phage JP4 with potential to control pathogenic Escherichia coli
af: Kexin Zhang (301752)
Udgivet: (2025)

Data Sheet 3_The impact of sublethal antimicrobial blue light on lipidomic changes of Escherichia coli and Salmonella Typhimurium.csv
af: Jiaxin Wu (540397)
Udgivet: (2025)

Data Sheet 1_The impact of sublethal antimicrobial blue light on lipidomic changes of Escherichia coli and Salmonella Typhimurium.pdf
af: Jiaxin Wu (540397)
Udgivet: (2025)

Data Sheet 2_The impact of sublethal antimicrobial blue light on lipidomic changes of Escherichia coli and Salmonella Typhimurium.csv
af: Jiaxin Wu (540397)
Udgivet: (2025)

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