HAstV-1 internalization and uncoating are delayed in FcRn-KO Caco-2 cells.

مواد مشابهة

• Mutations in the HAstV1/HAstV-8 chimeric virus were found in the FcRn-resistant variants.
  حسب: Catalina Aguilera-Flores (21743843)
  منشور في: (2025)
• HAstV infectivity and cell binding are blocked by preincubation with soluble FcRn.
  حسب: Catalina Aguilera-Flores (21743843)
  منشور في: (2025)
• HAstV-8 (D471S, N512D) spike has decreased or no binding to FcRn compared to wild-type HAstV-8 spike.
  حسب: Catalina Aguilera-Flores (21743843)
  منشور في: (2025)
• Working model for classical HAstV entry into Caco-2 cells.
  حسب: Catalina Aguilera-Flores (21743843)
  منشور في: (2025)
• Infectivity and binding of HAstV in wild-type, FcRn-KO, and DPP4-KO Caco-2 cells. A) Western blot showing the loss of expression of the FcRn receptor in FcRn-knockout (FcRn-KO), DPP4 in DPP4-KO cells, and of both cellular molecules in the double-KO Caco-2 cells. The arrow points to a nonspecific band revealed with the FcRn antibody. Tubulin was used as a loading control in the gel. B) Infectivity of HAst-V serotypes 1, 2, and 8, and of the divergent HAstV-VA1 in WT and three knockout Caco-2 cell lines. The infectious virus titers of HAstV-infected cell lysates were determined in Caco-2 cell monolayers grown in 96-well plates. C) Purified HAstV-1 binding in WT and the three knockout Caco-2 cell lines. Purified virus (160 ng) was added for 1 h at 4°C to allow the virus to attach to the cell surface. After removing the unbound virus and washing the cells, the attached virus was detected by RT-qPCR, as described in the Materials and Methods section. Experiments were performed on ice to
  حسب: Catalina Aguilera-Flores (21743843)
  منشور في: (2025)