Additional information of the RNG2 purification from insect cells.

(A) SEC chromatogram of the purified RNG2-ΔCTer variant obtained via Strep-tactin affinity purification, with Coomassie-stained analysis of the protein-containing fractions. (B) Western blot analysis of the purification of the ΔN+CTer and ΔCTer variants...

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Dades bibliogràfiques
Autor principal:	Romuald Haase (22339777) (author)
Altres autors:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Publicat:	2025
Matèries:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Sumari:	<p>(<b>A)</b> SEC chromatogram of the purified RNG2-ΔCTer variant obtained via Strep-tactin affinity purification, with Coomassie-stained analysis of the protein-containing fractions. <b>(B)</b> Western blot analysis of the purification of the ΔN+CTer and ΔCTer variants. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(C)</b> Mass spectrometry analysis identified peptides belonging to the purified RNG2ΔN+CTer and ΔCTer samples. <b>(D)</b> Overlay of chromatogram traces of four different RNG2 purifications: final sample of the RNG2 ∆N+CTer construct and isolated fragments of 60, 90 and 155 kDa fragment. Elution volume of reference proteins with defined MW is shown at the bottom (orange line). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p>

Additional information of the RNG2 purification from insect cells.

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