Analytical sensitivity and specificity of AS-qPCR assays targeting the deleted forms and their corresponding wild-type sequences.

<p>Cq values based on 10-fold serial dilutions of recombinant plasmid DNA ranging from 5x10<sup>7</sup> to 5 copies per reaction. Cq values for plasmids without amplification are set arbitrarily to 45. The plasmid copies are presented as logarithm to base 10 (log). R<sup>2<...

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Main Author:	Wajdi Ayadi (10315724) (author)
Other Authors:	Fahmi Smaoui (21246349) (author), Saba Gargouri (14325678) (author), Sana Ferjani (14325669) (author), Zaineb Hamzaoui (21246352) (author), Awatef Taktak (21246355) (author), Amel Chtourou (21246358) (author), Houda Skouri-Gargouri (21246361) (author), Azza Hadj Sassi (21246364) (author), Mouna Ben Sassi (21246367) (author), Sameh Trabelsi (5100557) (author), Ali Gargouri (3515591) (author), Ilhem Boutiba-Ben Boubaker (11740485) (author), Héla Karray-Hakim (21246370) (author), Raja Mokdad-Gargouri (11442455) (author), Lamia Feki-Berrajah (21246373) (author)
Published:	2025
Subjects:	Microbiology Genetics Molecular Biology Biotechnology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified Information Systems not elsewhere classified effective infection control 227 confirmed covid new relevant mutations especially omicron variants xlink "> monitoring verifying key sars simple approaches used existing lineages ba deletions &# 916 )- qpcr detection &# 916 mutations y264h cov2 variants additional variants xbb variant valuable tools specific qpcr sequencing results sequenced cases september 2022 second stage screening tests screening approach rigorously assessed restriction analysis report lessons recent sublineages rapid genotyping rapid detection quickly replaced proven methods predominant variant october 2022 methods appear major concern including ch identifying sars first stage findings highlight developing countries april 2023 analytical performance 7 %). 7 %) 62 ). 38 cases 19 cases 165 cases 14 cases 115 ),
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Analytical sensitivity and specificity of AS-qPCR assays targeting the deleted forms and their corresponding wild-type sequences.

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