Mosaic S-6P heterotrimer purification.

(A) SDS-PAGE gels with Coomassie blue staining from affinity purification of each S trimer. FT: Flow-through; Elu.: Elution (B) Western blot with anti-His primary antibody indicates the efficiency of HRV 3C protease digestion and tandem-affinity purific...

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Autor principal:	Chengbo Chen (11046348) (author)
Altres autors:	Klaus N. Lovendahl (22290965) (author), Julie M. Overbaugh (22290968) (author), Kelly K. Lee (9740516) (author)
Publicat:	2025
Matèries:	Biophysics Molecular Biology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified maintained without misfolding glycosylation profiles likewise exchange mass spectrometry evade immune responses bivalent mrna vaccines provide important information antigenically important regions xlink "> sars binding subunit compared specific dynamics similar polyvalent immunogen co enhanced immunogen display determined whether co mosaic spike trimers mosaic spike dynamics 2 variants results mosaic heterotrimer antigens assembly </ p spike antigens mosaic trimers important considerations mosaic heterotrimers understanding whether different sars multiple variants emerging variants 2 variants stabilizing effect retained protomer receptor binding preserved relative interprotomer crosstalk infection throughout homotrimer counterparts genetically close g614 heterotrimer epitope presentation
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Mosaic S-6P heterotrimer purification.

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