Critical Role of Transcription Factor CtSR in Mediating Ergosterol Biosynthesis and DMI Fungicides Sensitivity in Colletotrichum truncatum
Anthracnose caused by Colletotrichum truncatum severely impacts global fruit/vegetable yields. Unlike other Colletotrichum species, C. truncatum exhibits inherent resistance to some sterol demethylation inhibitors (DMIs), necessitating an understanding of ergosterol synthesis regulation and resistan...
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2025
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| الموضوعات: | |
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| الملخص: | Anthracnose caused by Colletotrichum truncatum severely impacts global fruit/vegetable yields. Unlike other Colletotrichum species, C. truncatum exhibits inherent resistance to some sterol demethylation inhibitors (DMIs), necessitating an understanding of ergosterol synthesis regulation and resistance mechanisms. A transcription factor, CtSR (CTRU02_06681), that regulates the DMI sensitivity in C. truncatum was identified in our study. Deletion of <i>CtSR</i> rendered a naturally resistant isolate highly sensitive to DMIs, significantly reduced total ergosterol levels, decreased <i>CYP51s</i> expression compared to the wild type, and abolished DMI-induced expression of <i>CYP51s</i>. Deletion of <i>CtSR</i> reduced mycelial growth and virulence and made the strain highly sensitive to oxidative stress but did not affect its sensitivity to osmotic stress. Transcriptomic analysis and yeast one-hybrid assays confirmed that CtSR directly binds the conserved promoter motif CGAATACGAA to regulate ergosterol biosynthesis genes. Taken together, our study demonstrates a critical role for CtSR in the regulation of ergosterol biosynthesis and DMI interactions in C. truncatum, which may have significant practical implications. |
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