Development of a sensitive HRM PCR analysis targeting the Strumpellin protein coding sequence.

(A) An HRM PCR assay was set up to be performed on a Light Cycler 480 Instrument. The analyses using the Gene scanning software allowed determining melting curves that are here represented in blue, red and green curves for the L. major (LEM3171: Tm = 89...

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محفوظ في:

التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Yusr Saadi-Ben Aoun (20469385) (author)
مؤلفون آخرون:	Hejer Souguir (11185775) (author), Hamed Chouaieb (11185769) (author), Mongia Kraiem (20469388) (author), Insaf Bel Hadj Ali (11185766) (author), Ahmed S. Chakroun (20469391) (author), Florian Noguier (20469394) (author), Akila Fathallah-Mili (11185790) (author), David Piquemal (85127) (author), Ikram Guizani (564611) (author)
منشور في:	2024
الموضوعات:	Medicine Microbiology Cell Biology Molecular Biology Cancer Infectious Diseases Virology Computational Biology Mathematical Sciences not elsewhere classified Physical Sciences not elsewhere classified molecular diagnostic algorithms improving disease management 62 %, respectively hrm profile interpretations assay could detect hrm could detect hrm pcr method human cutaneous samples hrm pcr assay hrm pcr target provides rapid results precise differentiation based accurate species identification pcr assay hrm pcr could serve three samples sequencing results pcr rflp effective target cutaneous leishmaniases based methods valuable tool tropica .</ traditionally relies three tests tarentolae </ strumpellin </ scale sample principle demonstration phylogenetic approaches parasite species old world nucleotide variations mediterranean region major </ leishmania </ evolutionary conservation effective diagnosis direct examination brought proof arabica </ analytical limit aethiopica </ 31 %. 3 genotypes
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Development of a sensitive HRM PCR analysis targeting the <i>Strumpellin</i> protein coding sequence.

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