LAI-1-dependent LCV remodeling requires GBP.
<p>(<b>A-C</b>) Dually labeled <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing calnexin (CnxA)-GFP (pAW016) and P4C-mCherry (pWS032) was treated with LAI-1 (10 µM, 1 h), or DMSO (solvent control), infected (MOI 5, 4 h) with mCerulean-producing <i>L....
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p>(<b>A-C</b>) Dually labeled <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing calnexin (CnxA)-GFP (pAW016) and P4C-mCherry (pWS032) was treated with LAI-1 (10 µM, 1 h), or DMSO (solvent control), infected (MOI 5, 4 h) with mCerulean-producing <i>L. pneumophila</i> JR32 (pNP99), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. (<b>D-G</b>) <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing CnxA-mCherry (pAW012) or P4C-mCherry (pWS032) was infected (MOI 5) for (<b>D</b>, <b>E</b>) 4 h or (<b>F</b>, <b>G</b>) 2-20 h with GFP-producing <i>L. pneumophila</i> JR32 harboring pMF16 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i>) or pMF17 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i><sup>K258A</sup>), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. The area of LCVs positive for (<b>E</b>, <b>F</b>) CnxA or (<b>E</b>, <b>G</b>) P4C was quantified using ImageJ software. Data shown are means and standard deviations of biological triplicates (Student’s t-test; *, <i>p</i> ≤ 0.05; **, <i>p</i> ≤ 0.01; ***, <i>p</i> ≤ 0.001).</p> |
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