LAI-1-dependent LCV remodeling requires GBP.
<p>(<b>A-C</b>) Dually labeled <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing calnexin (CnxA)-GFP (pAW016) and P4C-mCherry (pWS032) was treated with LAI-1 (10 µM, 1 h), or DMSO (solvent control), infected (MOI 5, 4 h) with mCerulean-producing <i>L....
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2025
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| _version_ | 1852020912298131456 |
|---|---|
| author | Franziska Solger (8826224) |
| author2 | Jonas Rauch (21213524) Simone Vormittag (17325505) Mingzhen Fan (12403234) Lyudmil Raykov (21213527) Paul Charki (18513863) Ana Katic (11137077) François Letourneur (21213530) Thierry Soldati (79025) Jürgen Seibel (2742718) Hubert Hilbi (72826) |
| author2_role | author author author author author author author author author author |
| author_facet | Franziska Solger (8826224) Jonas Rauch (21213524) Simone Vormittag (17325505) Mingzhen Fan (12403234) Lyudmil Raykov (21213527) Paul Charki (18513863) Ana Katic (11137077) François Letourneur (21213530) Thierry Soldati (79025) Jürgen Seibel (2742718) Hubert Hilbi (72826) |
| author_role | author |
| dc.creator.none.fl_str_mv | Franziska Solger (8826224) Jonas Rauch (21213524) Simone Vormittag (17325505) Mingzhen Fan (12403234) Lyudmil Raykov (21213527) Paul Charki (18513863) Ana Katic (11137077) François Letourneur (21213530) Thierry Soldati (79025) Jürgen Seibel (2742718) Hubert Hilbi (72826) |
| dc.date.none.fl_str_mv | 2025-04-29T18:18:03Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013026.g007 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/LAI-1-dependent_LCV_remodeling_requires_GBP_/28898372 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Cell Biology Genetics Molecular Biology Ecology Developmental Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuoles harboring avirulent resistant environmental bacterium lipid droplet markers legionnaires ’ disease div >< p efficient intracellular replication lcv markers calnexin hydroxyketone compound lai 1 derivatives inhibit dictyostelium discoideum </ legionella pneumophila </ kingdom signaling involves kingdom signaling pneumophila </ legionella </ discoideum </ intracellular growth gnbp </ 1 involves work shows taken together synthetic lai single guanylate replicates intracellularly mutually dependent mcs ). mammalian cells lcv localization lcv ). large gtpases distinct compartment dependent manner dependent inhibition causative agent binding protein antimicrobial factor 1 colocalizes 1 (< |
| dc.title.none.fl_str_mv | LAI-1-dependent LCV remodeling requires GBP. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(<b>A-C</b>) Dually labeled <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing calnexin (CnxA)-GFP (pAW016) and P4C-mCherry (pWS032) was treated with LAI-1 (10 µM, 1 h), or DMSO (solvent control), infected (MOI 5, 4 h) with mCerulean-producing <i>L. pneumophila</i> JR32 (pNP99), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. (<b>D-G</b>) <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing CnxA-mCherry (pAW012) or P4C-mCherry (pWS032) was infected (MOI 5) for (<b>D</b>, <b>E</b>) 4 h or (<b>F</b>, <b>G</b>) 2-20 h with GFP-producing <i>L. pneumophila</i> JR32 harboring pMF16 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i>) or pMF17 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i><sup>K258A</sup>), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. The area of LCVs positive for (<b>E</b>, <b>F</b>) CnxA or (<b>E</b>, <b>G</b>) P4C was quantified using ImageJ software. Data shown are means and standard deviations of biological triplicates (Student’s t-test; *, <i>p</i> ≤ 0.05; **, <i>p</i> ≤ 0.01; ***, <i>p</i> ≤ 0.001).</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_be697ecda749bbd440c0c848c8bacc7f |
| identifier_str_mv | 10.1371/journal.ppat.1013026.g007 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28898372 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | LAI-1-dependent LCV remodeling requires GBP.Franziska Solger (8826224)Jonas Rauch (21213524)Simone Vormittag (17325505)Mingzhen Fan (12403234)Lyudmil Raykov (21213527)Paul Charki (18513863)Ana Katic (11137077)François Letourneur (21213530)Thierry Soldati (79025)Jürgen Seibel (2742718)Hubert Hilbi (72826)BiochemistryCell BiologyGeneticsMolecular BiologyEcologyDevelopmental BiologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedvacuoles harboring avirulentresistant environmental bacteriumlipid droplet markerslegionnaires ’ diseasediv >< pefficient intracellular replicationlcv markers calnexinhydroxyketone compound lai1 derivatives inhibitdictyostelium discoideum </legionella pneumophila </kingdom signaling involveskingdom signalingpneumophila </legionella </discoideum </intracellular growthgnbp </1 involveswork showstaken togethersynthetic laisingle guanylatereplicates intracellularlymutually dependentmcs ).mammalian cellslcv localizationlcv ).large gtpasesdistinct compartmentdependent mannerdependent inhibitioncausative agentbinding proteinantimicrobial factor1 colocalizes1 (<<p>(<b>A-C</b>) Dually labeled <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing calnexin (CnxA)-GFP (pAW016) and P4C-mCherry (pWS032) was treated with LAI-1 (10 µM, 1 h), or DMSO (solvent control), infected (MOI 5, 4 h) with mCerulean-producing <i>L. pneumophila</i> JR32 (pNP99), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. (<b>D-G</b>) <i>D. discoideum</i> Ax2 or Δ<i>gnbp</i> producing CnxA-mCherry (pAW012) or P4C-mCherry (pWS032) was infected (MOI 5) for (<b>D</b>, <b>E</b>) 4 h or (<b>F</b>, <b>G</b>) 2-20 h with GFP-producing <i>L. pneumophila</i> JR32 harboring pMF16 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i>) or pMF17 (P<sub><i>6SRNA</i></sub>-<i>lqsA</i><sup>K258A</sup>), fixed and analyzed by confocal microscopy. Scale bars, 3 µm. The area of LCVs positive for (<b>E</b>, <b>F</b>) CnxA or (<b>E</b>, <b>G</b>) P4C was quantified using ImageJ software. Data shown are means and standard deviations of biological triplicates (Student’s t-test; *, <i>p</i> ≤ 0.05; **, <i>p</i> ≤ 0.01; ***, <i>p</i> ≤ 0.001).</p>2025-04-29T18:18:03ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013026.g007https://figshare.com/articles/figure/LAI-1-dependent_LCV_remodeling_requires_GBP_/28898372CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/288983722025-04-29T18:18:03Z |
| spellingShingle | LAI-1-dependent LCV remodeling requires GBP. Franziska Solger (8826224) Biochemistry Cell Biology Genetics Molecular Biology Ecology Developmental Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuoles harboring avirulent resistant environmental bacterium lipid droplet markers legionnaires ’ disease div >< p efficient intracellular replication lcv markers calnexin hydroxyketone compound lai 1 derivatives inhibit dictyostelium discoideum </ legionella pneumophila </ kingdom signaling involves kingdom signaling pneumophila </ legionella </ discoideum </ intracellular growth gnbp </ 1 involves work shows taken together synthetic lai single guanylate replicates intracellularly mutually dependent mcs ). mammalian cells lcv localization lcv ). large gtpases distinct compartment dependent manner dependent inhibition causative agent binding protein antimicrobial factor 1 colocalizes 1 (< |
| status_str | publishedVersion |
| title | LAI-1-dependent LCV remodeling requires GBP. |
| title_full | LAI-1-dependent LCV remodeling requires GBP. |
| title_fullStr | LAI-1-dependent LCV remodeling requires GBP. |
| title_full_unstemmed | LAI-1-dependent LCV remodeling requires GBP. |
| title_short | LAI-1-dependent LCV remodeling requires GBP. |
| title_sort | LAI-1-dependent LCV remodeling requires GBP. |
| topic | Biochemistry Cell Biology Genetics Molecular Biology Ecology Developmental Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuoles harboring avirulent resistant environmental bacterium lipid droplet markers legionnaires ’ disease div >< p efficient intracellular replication lcv markers calnexin hydroxyketone compound lai 1 derivatives inhibit dictyostelium discoideum </ legionella pneumophila </ kingdom signaling involves kingdom signaling pneumophila </ legionella </ discoideum </ intracellular growth gnbp </ 1 involves work shows taken together synthetic lai single guanylate replicates intracellularly mutually dependent mcs ). mammalian cells lcv localization lcv ). large gtpases distinct compartment dependent manner dependent inhibition causative agent binding protein antimicrobial factor 1 colocalizes 1 (< |