Cytosine non-conversion within and between reads on unmethylated DNA from A. thaliana, D. melanogaster and phage .

Cytosine non-conversion within and between reads on unmethylated DNA from A. thaliana, D. melanogaster and phage .

<p>For <i>A. thaliana</i>, data are shown for reads aligning to the first 75 kb of chloroplast. ( <b>A</b>) Overall cytosine non-conversion rates in libraries prepared using different relative concentrations of Tn5 transposase to DNA, and 13 or 15 PCR cycles. ( <b>...

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Bibliographic Details
Main Author: Thomas James Ellis (21403016) (author)
Other Authors: Viktoria Nizhynska (357986) (author), Rahul Pisupati (3852202) (author), Almudena Mollá-Morales (21403019) (author), Magnus Nordborg (16566) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Genetics
Molecular Biology
Mental Health
Biological Sciences not elsewhere classified
Physical Sciences not elsewhere classified
xlink ">&# 8216
tagmentation &# 8217
simultaneously make double
less starting material
impede biological conclusions
certain tagmentation protocols
single strand nicks
bisulphite sequencing use
bisulphite sequencing
strand displacement
stranded breaks
resulting fragments
python package
methylated cytosines
ligate adaptors
higher throughput
erroneous incorporation
downstream analysis
dna fragment
allow users
additional effort
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