Cytosine non-conversion within and between reads on unmethylated DNA from A. thaliana, D. melanogaster and phage .
<p>For <i>A. thaliana</i>, data are shown for reads aligning to the first 75 kb of chloroplast. ( <b>A</b>) Overall cytosine non-conversion rates in libraries prepared using different relative concentrations of Tn5 transposase to DNA, and 13 or 15 PCR cycles. ( <b>...
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2025
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| Summary: | <p>For <i>A. thaliana</i>, data are shown for reads aligning to the first 75 kb of chloroplast. ( <b>A</b>) Overall cytosine non-conversion rates in libraries prepared using different relative concentrations of Tn5 transposase to DNA, and 13 or 15 PCR cycles. ( <b>B</b>) The distribution of the proportion of unconverted cytosines across reads. For clarity, reads with no unconverted cytosines are not shown. ( <b>C</b>) The probability of observing an unconverted cytosine at each base0pair position within reads each of the four strands generated by bisulphite libraries (OT=original top strand; OB=original bottom; CTOT, complementary to original top; CTOB, complementary to original bottom) averaged over libraries.</p> |
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