One-Step Labeling and Purification: Accelerating FAHFA Detection in Vegetable Oils via Chemoselective Probe-Assisted LC–MS

One-Step Labeling and Purification: Accelerating FAHFA Detection in Vegetable Oils via Chemoselective Probe-Assisted LC–MS

Fatty acid esters of hydroxy fatty acids (FAHFAs) are emerging bioactive lipids in edible oils, but their trace-level presence and matrix complexity hinder accurate analysis. Here, we designed a novel chemoselective probe piperazine-modified silica (<b>SiO</b><sub><b>2</b&...

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Bibliographic Details
Main Author: Xin-Ze Wu (16624862) (author)
Other Authors: Kai-Wen Tan (21793250) (author), Xu Zhang (49484) (author), Jun-Jie Tan (6245813) (author), Na An (299087) (author), Fang Cong (16757832) (author), Wenbin Wu (292223) (author), Azamat Temerdashev (21793253) (author), Quan-Fei Zhu (4852795) (author), Hong Wang (13528) (author), Yu-Qi Feng (438357) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Neuroscience
Biotechnology
Inorganic Chemistry
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vegetable oil quality
hydroxy fatty acids
five dominant isomers
emerging bioactive lipids
accelerating fahfa detection
76 × 10
47 fahfa isomers
probe enabled single
peanut oils exhibiting
highest fahfa concentrations
step labeling reaction
specifically captures fahfas
g ., 10
4 </ sup
0039 – 0
step labeling
enabled quantification
edible oils
concentrations ranging
traditional methods
significantly enhancing
signal amplification
promising platform
level presence
integrating capture
effectively overcoming
approach provides
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