Phage susceptibility of strains expressing chimeric SLCT-L6/H4 and SLCT-L4/H6.

<p>Spot tests on FM2.5 and R20291 Δ<i>slpA</i> strains complemented with SLCT-L6/H4 and SLCT-L4/H6, respectively. Serial 10-fold dilutions of the indicated phages (undiluted titers >10⁸ PFU/mL) were spotted onto bacterial lawns. Infections confirmed as positive are highlighted i...

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1. autor:	Alexia L.M. Royer (22693792) (author)
Kolejni autorzy:	Émeline Dion (22693795) (author), Andrew A. Umansky (22693798) (author), Mariano Avino (243049) (author), Olga Soutourina (248942) (author), Louis-Charles Fortier (225877) (author)
Wydane:	2025
Hasła przedmiotowe:	Biochemistry Microbiology Molecular Biology Biotechnology Ecology Immunology Cancer Infectious Diseases Virology Biological Sciences not elsewhere classified remains poorly understood provide new insights div >< p creating chimeric constructs expressing modified isoforms phages adsorbed efficiently layer protein contribute lmw d2 domain govern phage binding clostridioides difficile </ mediated phage recognition general phage receptor phage infection cycle layer protein slpa layer protein receptor binding difficile </ phage recognition different isoforms successful infection slpa </ several phages seven phages productive infection precise recognition phage therapy phage specificity phage cocktails structural features structural determinants slpa required results demonstrate r20291 δ promising alternative previously identified molecular determinants host receptor hmw fragments highlighting differences future design fundamental importance frequently required dependent manner delayed adsorption critical step broad spectrum always predictive always essential also able
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Phage susceptibility of strains expressing chimeric SLCT-L6/H4 and SLCT-L4/H6.

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