Denaturing PAGE analysis of the cleavage products following the action of TDGcat and TDGFL on the regular 41-mer 14–03 A•T* dmbDNA substrate.

After the reaction, samples were either treated or not with hot alkali. Lane 1: control 14–03 dmbDNA with 5′-32P-labelled bottom 55-mer strand; lanes 2–3, same as lane 1 but with TDGcat, no hot alkali treatment; lanes 4–5, same as lanes 2–3, but...

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Bibliographic Details
Main Author:	Diana Manapkyzy (19871017) (author)
Other Authors:	Botagoz Joldybayeva (542447) (author), Alexander A. Ishchenko (9693725) (author), Bakhyt T. Matkarimov (19871020) (author), Dmitry O. Zharkov (542449) (author), Sabira Taipakova (9693716) (author), Murat K. Saparbaev (345036) (author)
Published:	2024
Subjects:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Developmental Biology Cancer turnover conditions shows isolated catalytic domain experimental conditions used cleavage product accumulation apparent rate constant 85 – 330 extended incubation time active dna demethylation sup >&# 8722 47 – 0 0014 – 0 specific dna duplex damaged dna duplex div >< p cpg binding domain containing protein 4 37 ° c regular dna duplexes fl </ sup base excision repair • g mispairs duplex dna vivo </ time course base pairs dna glycosylase terminal domains strand breaks regular non physiologically relevant overall conformation mitotic cells methylcytosine residues human thymine gene expression fold lower equimolar concentrations epigenetic regulation disordered n dependent removal cpg contexts cpg context also involved 61 min 5hmc residues 5hmc ), 0069 min
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Denaturing PAGE analysis of the cleavage products following the action of TDG<sup>cat</sup> and TDG<sup>FL</sup> on the regular 41-mer 14–03 A•T* dmbDNA substrate.

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