Occlusion of a C-terminal NCAPH2 degron explains context-dependent effects of tag fusion.

Occlusion of a C-terminal NCAPH2 degron explains context-dependent effects of tag fusion.

<p>A. Genome editing strategy to integrate a C-terminal GFP fusion at the endogenous <i><i>Ncaph2</i></i> locus in mouse embryonic stem cells. sgRNAs cut the genome in the terminal intron and 3’UTR of the endogenous <i><i>Ncaph2</i></i> gene, and...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Gillian C. A. Taylor (3909568) (author)
مؤلفون آخرون: Lewis Macdonald (22120476) (author), Natalia A. Szulc (12733802) (author), Evelina Gudauskaite (22120479) (author), Brianda Hernandez Moran (22120482) (author), Jennifer M. Brisbane (22120485) (author), Molly Donald (22120488) (author), Ella Taylor (22120491) (author), Dejin Zheng (22120494) (author), Bin Gu (223694) (author), Pleasantine Mill (256953) (author), Patricia L. Yeyati (22120497) (author), Wojciech Pokrzywa (37845) (author), Claudia Ribeiro de Almeida (16007018) (author), Andrew J. Wood (22120500) (author)
منشور في: 2025
الموضوعات:
Biophysics
Biochemistry
Microbiology
Genetics
Molecular Biology
Physiology
Biotechnology
Developmental Biology
Cancer
Inorganic Chemistry
Infectious Diseases
Plant Biology
Virology
Biological Sciences not elsewhere classified
Physical Sciences not elsewhere classified
one case (<
mitotic cells via
cause unwanted changes
trigger protein degradation
study protein functions
div >< p
terminal tag fusion
dependent changes
cultured cells
tagged protein
tag fusion
protein tags
protein stability
protein expression
genetic fusion
widely used
well known
vivo </
underappreciated importance
tir1 expression
terminal auxin
tag fusions
stabilisation occurred
small intestine
results highlight
reach levels
particularly important
occurred selectively
ncaph2 </
inherent property
inducible degron
destabilisation resulted
basal concentrations
animal models
acetic acid
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