Expression of COWP3-mNeon, COWP4-mNeon and COWP5-mNeon tagged strains at <i>CpIMPDH</i> locus.

Expression of COWP3-mNeon, COWP4-mNeon and COWP5-mNeon tagged strains at <i>CpIMPDH</i> locus.

<p>A) Promoter and full open reading frame (ORF) of <i>COWP3</i>, <i>COWP4</i> and <i>COWP5</i> each cloned with C-terminal mNeonGreen to generate COWP3/4/5-mNeon repair cassette. Each repair cassette was targeted for integration at <i>Cpimpdh</i>...

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Bibliographic Details
Main Author:	Ross Bacchetti (16752855) (author)
Other Authors:	Sarah Stevens (398830) (author), Leandro Lemgruber (2882552) (author), Mariana Azevedo Gonzalez Oliva (22486492) (author), Emma M. Sands (16752861) (author), Konstantinos Alexandrou (22486495) (author), Michele Tinti (75217) (author), Lee Robinson (9651716) (author), Jack C. Hanna (16752846) (author), Simona Seizova (16752852) (author), Peyton Goddard (22486498) (author), Massimo Vassalli (2178036) (author), Mattie Christine Pawlowic (22486501) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </
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