The nsp15 mutations increase the sensitivity to temperature and exogenous IFN-β.

<p>(A) Temperature-dependent replication of WT and nsp15-mutant HCoV-229E viruses in IFN responsive (HEL299) or non-responsive (Huh7-STAT1-KO) cells. The graphs show the mean CCID<sub>50</sub> values of two independent virus titration experiments, conducted at the indicated tempera...

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Main Author:	Benjamin Van Loy (9496075) (author)
Other Authors:	Eugènia Pujol (20707338) (author), Kenichi Kamata (10077986) (author), Xiao Yin Lee (20707341) (author), Nikolai Bakirtzoglou (20707344) (author), Ria Van Berwaer (10686932) (author), Julie Vandeput (10676491) (author), Cato Mestdagh (20707347) (author), Leentje Persoons (333605) (author), Brent De Wijngaert (20707350) (author), Quinten Goovaerts (20707353) (author), Sam Noppen (253207) (author), Maarten Jacquemyn (10097515) (author), Kourosh Ahmadzadeh (10296365) (author), Eline Bernaerts (11050593) (author), Juan Martín-López (20707356) (author), Celia Escriche (20707359) (author), Bert Vanmechelen (4540189) (author), Besir Krasniqi (6336584) (author), Abhimanyu K. Singh (8624646) (author), Dirk Daelemans (397676) (author), Piet Maes (473809) (author), Patrick Matthys (12567) (author), Wim Dehaen (496141) (author), Jef Rozenski (57476) (author), Kalyan Das (1338543) (author), Arnout Voet (111769) (author), Santiago Vázquez (2691265) (author), Lieve Naesens (333606) (author), Annelies Stevaert (1665184) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Genetics Molecular Biology Pharmacology Biotechnology Immunology Cancer Science Policy Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified mainly act via catalytic his250 residue human fibroblast cells novel zoonotic coronaviruses carried resistance sites viral rna synthesis substituted diphenylurea compound impair compound binding virus escapes epb recombinant nsp15 proteins human coronavirus 229e distinct antiviral mechanism higher endou activity endou cleavage activity suppresses cov replication human macrophages resistance profile also suppresses endou activity viral non viral dsrna antiviral targeting antiviral drugs mechanism explains independent mechanism appealing mechanism endou domain xlink "> thermal stability specific endoribonuclease resistant variants readily outcompeted proved unable nsp15 endoribonuclease nsp15 ). novel guanidine known function hexameric nsp15 future outbreaks findings suggest exhibits strong enzymatic assays designated epb conformational change avoid induction 19 vaccines
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The nsp15 mutations increase the sensitivity to temperature and exogenous IFN-β.

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