CYP46A1 and 24-OHC are up-regulated in PD patients and PD model mice.

CYP46A1 and 24-OHC are up-regulated in PD patients and PD model mice.

<p><b> (A)</b> Immunohistochemistry showing the levels of CYP46A1 in the SNpc of PD patients (<i>n</i> = 5 subjects in each group). Scale bar, 20 μm. <b>(B)</b> Levels of 24-OHC in the plasma of PD patients (<i>n</i> = 38) and control subjects (&...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Lijun Dai (2407099) (author)
مؤلفون آخرون: Jiannan Wang (154792) (author), Lanxia Meng (564023) (author), Xingyu Zhang (408987) (author), Tingting Xiao (838381) (author), Min Deng (114029) (author), Guiqin Chen (5955809) (author), Jing Xiong (218613) (author), Wei Ke (158476) (author), Zhengyuan Hong (20746042) (author), Lihong Bu (443666) (author), Zhentao Zhang (218622) (author)
منشور في: 2025
الموضوعات:
Biochemistry
Cell Biology
Neuroscience
Science Policy
Biological Sciences not elsewhere classified
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parkinson &# 8217
mitochondrial dysfunction induced
activation gene 3
nigrostriatal dopaminergic degeneration
neurodegenerative disease characterized
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ohc promote neurotoxicity
disease </ p
dopaminergic neurons
substantia nigra
strategies aimed
specific oxysterol
ohc exacerbates
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mouse model
modifying therapies
lewy bodies
intracerebral injection
cerebrospinal fluid
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الوصف
الملخص:<p><b> (A)</b> Immunohistochemistry showing the levels of CYP46A1 in the SNpc of PD patients (<i>n</i> = 5 subjects in each group). Scale bar, 20 μm. <b>(B)</b> Levels of 24-OHC in the plasma of PD patients (<i>n</i> = 38) and control subjects (<i>n</i> = 19) determined by LC–MS. Student’s <i>t</i> test with Welch’s correction. **<i>P</i> < 0.01. <b>(C)</b> Representative immunohistochemistry images of CYP46A1 in the cortex and SN of α-Syn A53T transgenic mice at different ages. Scale bar, 20 μm. Bar graphs are the optical density (<i>n</i> = 5 mice per group). <b>(D)</b> Immunoblotting showing CYP46A1 levels in the striatal lysates of α-Syn A53T transgenic mice at different ages. GAPDH was used as the loading control (<i>n</i> = 6 mice per group). <b>(E)</b> LC–MS analysis of plasma 24-OHC levels in α-Syn A53T transgenic mice at different ages (<i>n</i> = 4–5 mice per group). <b>(F)</b> LC–MS analysis of 24-OHC levels in the striatal lysates of α-Syn A53T transgenic mice at different ages (<i>n</i> = 4–5 mice per group). All data are means ± SEM. One-way ANOVA with Tukey’s multiple comparisons test. *<i>P</i> < 0.05, ****<i>P</i> < 0.001, ****<i>P</i> < 0.0001, and ns, not significant. Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002974#pbio.3002974.s011" target="_blank">S1 Data</a>. The uncropped blots are included in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002974#pbio.3002974.s012" target="_blank">S1 Raw</a> Images. PD, Parkinson’s disease; SN, substantia nigra; SNpc, substantia nigra pars compacta.</p>

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