Discontinuous transcription in coronaviruses.

<p><b>(A)</b> Schematic of the SARS-CoV-2 genome and <b>(B)</b> the mechanism of discontinuous transcription for expression of the structural (S, E, M, N) and accessory genes (yellow highlight). <b>(C)</b> Alignment of TRS-Bs upstream of each of the indicate...

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Main Author: Harriet V. Mears (17873605) (author)
Other Authors: George R. Young (8893355) (author), Theo Sanderson (5662720) (author), Ruth Harvey (323318) (author), Jamie Barrett-Rodger (20592217) (author), Rebecca Penn (9979944) (author), Vanessa Cowton (13189782) (author), Wilhelm Furnon (10196183) (author), Giuditta De Lorenzo (552196) (author), Margaret Crawford (11903252) (author), Daniel M. Snell (20592223) (author), Ashley S. Fowler (17873602) (author), Anob M. Chakrabarti (20144324) (author), Saira Hussain (8268135) (author), Ciarán Gilbride (20592226) (author), Edward Emmott (493404) (author), Katja Finsterbusch (544282) (author), Jakub Luptak (9415797) (author), Thomas P. Peacock (6536120) (author), Jérôme Nicod (214878) (author), Arvind H. Patel (10196255) (author), Massimo Palmarini (81656) (author), Emma Wall (12895910) (author), Bryan Williams (4283011) (author), Sonia Gandhi (49260) (author), Charles Swanton (236153) (author), David L. V. Bauer (17873608) (author)
Published: 2025
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author Harriet V. Mears (17873605)
author2 George R. Young (8893355)
Theo Sanderson (5662720)
Ruth Harvey (323318)
Jamie Barrett-Rodger (20592217)
Rebecca Penn (9979944)
Vanessa Cowton (13189782)
Wilhelm Furnon (10196183)
Giuditta De Lorenzo (552196)
Margaret Crawford (11903252)
Daniel M. Snell (20592223)
Ashley S. Fowler (17873602)
Anob M. Chakrabarti (20144324)
Saira Hussain (8268135)
Ciarán Gilbride (20592226)
Edward Emmott (493404)
Katja Finsterbusch (544282)
Jakub Luptak (9415797)
Thomas P. Peacock (6536120)
Jérôme Nicod (214878)
Arvind H. Patel (10196255)
Massimo Palmarini (81656)
Emma Wall (12895910)
Bryan Williams (4283011)
Sonia Gandhi (49260)
Charles Swanton (236153)
David L. V. Bauer (17873608)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author_facet Harriet V. Mears (17873605)
George R. Young (8893355)
Theo Sanderson (5662720)
Ruth Harvey (323318)
Jamie Barrett-Rodger (20592217)
Rebecca Penn (9979944)
Vanessa Cowton (13189782)
Wilhelm Furnon (10196183)
Giuditta De Lorenzo (552196)
Margaret Crawford (11903252)
Daniel M. Snell (20592223)
Ashley S. Fowler (17873602)
Anob M. Chakrabarti (20144324)
Saira Hussain (8268135)
Ciarán Gilbride (20592226)
Edward Emmott (493404)
Katja Finsterbusch (544282)
Jakub Luptak (9415797)
Thomas P. Peacock (6536120)
Jérôme Nicod (214878)
Arvind H. Patel (10196255)
Massimo Palmarini (81656)
Emma Wall (12895910)
Bryan Williams (4283011)
Sonia Gandhi (49260)
Charles Swanton (236153)
David L. V. Bauer (17873608)
author_role author
dc.creator.none.fl_str_mv Harriet V. Mears (17873605)
George R. Young (8893355)
Theo Sanderson (5662720)
Ruth Harvey (323318)
Jamie Barrett-Rodger (20592217)
Rebecca Penn (9979944)
Vanessa Cowton (13189782)
Wilhelm Furnon (10196183)
Giuditta De Lorenzo (552196)
Margaret Crawford (11903252)
Daniel M. Snell (20592223)
Ashley S. Fowler (17873602)
Anob M. Chakrabarti (20144324)
Saira Hussain (8268135)
Ciarán Gilbride (20592226)
Edward Emmott (493404)
Katja Finsterbusch (544282)
Jakub Luptak (9415797)
Thomas P. Peacock (6536120)
Jérôme Nicod (214878)
Arvind H. Patel (10196255)
Massimo Palmarini (81656)
Emma Wall (12895910)
Bryan Williams (4283011)
Sonia Gandhi (49260)
Charles Swanton (236153)
David L. V. Bauer (17873608)
dc.date.none.fl_str_mv 2025-01-21T18:40:22Z
dc.identifier.none.fl_str_mv 10.1371/journal.pbio.3002982.g001
dc.relation.none.fl_str_mv https://figshare.com/articles/figure/Discontinuous_transcription_in_coronaviruses_/28250410
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Medicine
Microbiology
Genetics
Molecular Biology
Evolutionary Biology
Cancer
Mental Health
Infectious Diseases
Virology
Computational Biology
undergoing evolutionary changes
orf1b &# 8212
observe distinct phenotypes
functional rna level
accessory genes via
5 &# 8242
3 &# 8242
transcription regulatory sequences
nsp16 coding region
supported examples upstream
enhance viral fitness
nucleocapsid coding sequence
2 subgenomic rnas
coding region
viral fitness
subgenomic rnas
2 sequences
whose synthesis
upon searching
truncated c
terminal portion
show well
nucleocapsid gene
mutated compared
multiple lineages
interferon production
genomic leader
frequent neo
evolves frequently
evolved convergently
descendants thereof
consensus aaacgaac
concern alpha
dc.title.none.fl_str_mv Discontinuous transcription in coronaviruses.
dc.type.none.fl_str_mv Image
Figure
info:eu-repo/semantics/publishedVersion
image
description <p><b>(A)</b> Schematic of the SARS-CoV-2 genome and <b>(B)</b> the mechanism of discontinuous transcription for expression of the structural (S, E, M, N) and accessory genes (yellow highlight). <b>(C)</b> Alignment of TRS-Bs upstream of each of the indicated ORFs, compared to the TRS-L. Regions of homology to the TRS-L are highlighted in blue and downstream homology to the 5′UTR is highlighted in green. TRS, transcription regulatory sequence.</p>
eu_rights_str_mv openAccess
id Manara_cd873d3f0fbb5a2d9fd38564b939941f
identifier_str_mv 10.1371/journal.pbio.3002982.g001
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/28250410
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Discontinuous transcription in coronaviruses.Harriet V. Mears (17873605)George R. Young (8893355)Theo Sanderson (5662720)Ruth Harvey (323318)Jamie Barrett-Rodger (20592217)Rebecca Penn (9979944)Vanessa Cowton (13189782)Wilhelm Furnon (10196183)Giuditta De Lorenzo (552196)Margaret Crawford (11903252)Daniel M. Snell (20592223)Ashley S. Fowler (17873602)Anob M. Chakrabarti (20144324)Saira Hussain (8268135)Ciarán Gilbride (20592226)Edward Emmott (493404)Katja Finsterbusch (544282)Jakub Luptak (9415797)Thomas P. Peacock (6536120)Jérôme Nicod (214878)Arvind H. Patel (10196255)Massimo Palmarini (81656)Emma Wall (12895910)Bryan Williams (4283011)Sonia Gandhi (49260)Charles Swanton (236153)David L. V. Bauer (17873608)MedicineMicrobiologyGeneticsMolecular BiologyEvolutionary BiologyCancerMental HealthInfectious DiseasesVirologyComputational Biologyundergoing evolutionary changesorf1b &# 8212observe distinct phenotypesfunctional rna levelaccessory genes via5 &# 82423 &# 8242transcription regulatory sequencesnsp16 coding regionsupported examples upstreamenhance viral fitnessnucleocapsid coding sequence2 subgenomic rnascoding regionviral fitnesssubgenomic rnas2 sequenceswhose synthesisupon searchingtruncated cterminal portionshow wellnucleocapsid genemutated comparedmultiple lineagesinterferon productiongenomic leaderfrequent neoevolves frequentlyevolved convergentlydescendants thereofconsensus aaacgaacconcern alpha<p><b>(A)</b> Schematic of the SARS-CoV-2 genome and <b>(B)</b> the mechanism of discontinuous transcription for expression of the structural (S, E, M, N) and accessory genes (yellow highlight). <b>(C)</b> Alignment of TRS-Bs upstream of each of the indicated ORFs, compared to the TRS-L. Regions of homology to the TRS-L are highlighted in blue and downstream homology to the 5′UTR is highlighted in green. TRS, transcription regulatory sequence.</p>2025-01-21T18:40:22ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3002982.g001https://figshare.com/articles/figure/Discontinuous_transcription_in_coronaviruses_/28250410CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/282504102025-01-21T18:40:22Z
spellingShingle Discontinuous transcription in coronaviruses.
Harriet V. Mears (17873605)
Medicine
Microbiology
Genetics
Molecular Biology
Evolutionary Biology
Cancer
Mental Health
Infectious Diseases
Virology
Computational Biology
undergoing evolutionary changes
orf1b &# 8212
observe distinct phenotypes
functional rna level
accessory genes via
5 &# 8242
3 &# 8242
transcription regulatory sequences
nsp16 coding region
supported examples upstream
enhance viral fitness
nucleocapsid coding sequence
2 subgenomic rnas
coding region
viral fitness
subgenomic rnas
2 sequences
whose synthesis
upon searching
truncated c
terminal portion
show well
nucleocapsid gene
mutated compared
multiple lineages
interferon production
genomic leader
frequent neo
evolves frequently
evolved convergently
descendants thereof
consensus aaacgaac
concern alpha
status_str publishedVersion
title Discontinuous transcription in coronaviruses.
title_full Discontinuous transcription in coronaviruses.
title_fullStr Discontinuous transcription in coronaviruses.
title_full_unstemmed Discontinuous transcription in coronaviruses.
title_short Discontinuous transcription in coronaviruses.
title_sort Discontinuous transcription in coronaviruses.
topic Medicine
Microbiology
Genetics
Molecular Biology
Evolutionary Biology
Cancer
Mental Health
Infectious Diseases
Virology
Computational Biology
undergoing evolutionary changes
orf1b &# 8212
observe distinct phenotypes
functional rna level
accessory genes via
5 &# 8242
3 &# 8242
transcription regulatory sequences
nsp16 coding region
supported examples upstream
enhance viral fitness
nucleocapsid coding sequence
2 subgenomic rnas
coding region
viral fitness
subgenomic rnas
2 sequences
whose synthesis
upon searching
truncated c
terminal portion
show well
nucleocapsid gene
mutated compared
multiple lineages
interferon production
genomic leader
frequent neo
evolves frequently
evolved convergently
descendants thereof
consensus aaacgaac
concern alpha