Multiple epitopes-tagging across RNG2.

Multiple epitopes-tagging across RNG2.

<p><b>(A) Upper panel:</b> schematic representation of the modified endogenous locus. <b>Lower panel:</b> polymerase chain reaction of genomic extracted DNA from <i>Toxoplasma gondii</i> tachyzoites to verify the insertion of the N-terminal Myc tag/Internal...

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Bibliographic Details
Main Author: Romuald Haase (22339777) (author)
Other Authors: Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Physiology
Infectious Diseases
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Physical Sciences not elsewhere classified
toxoplasma gondii </
remains apically anchored
heterologous expression systems
div >< p
apical polar ring
biochemical analysis revealed
proper conoid anchorage
ensures conoid functionality
less parasites revealed
host cell invasion
enable parasite motility
controlling parasite motility
although rng2 depletion
intact conoid organelle
organelle traverses
intact protein
conditional depletion
biochemical properties
detached conoid
gliding motility
unstable protein
undergoes extrusion
two structures
strong candidate
striking detachment
still unclear
rng2 tethers
rng2 led
rng2 emerges
rhoptries follow
resilient bridge
protein localized
plasma membrane
pivotal protein
likely facilitate
large coiled
immunoelectron microscopy
functional activity
enabling rng2
electron tomography
dependent extrusion
critical role
concatenated assemblies
comprehensive mutagenesis
coil domains
calcium waves
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