A novel data processing method <i>CyC</i>* for quantitative real time polymerase chain reaction minimizes cumulative error

A novel data processing method <i>CyC</i>* for quantitative real time polymerase chain reaction minimizes cumulative error

<div><p>Quantitative real-time polymerase chain reaction (qPCR) is routinely conducted for DNA quantitative analysis using the cycle-threshold (<i>Ct</i>) method, which assumes uniform/optimum template amplification. In practice, amplification efficiencies vary from cycle to...

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Bibliographic Details
Main Author: Linzhong Zhang (6824447) (author)
Other Authors: Rui Dong (432995) (author), Shu Wei (845364) (author), Han-Chen Zhou (6824450) (author), Meng-Xian Zhang (6824453) (author), Karthikeyan Alagarsamy (6824456) (author)
Published: 2019
Subjects:
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Biological Sciences not elsewhere classified
Mathematical Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
fluorescence intensity F 0
novel data processing method CyC
template
outlier cycle C
amplification efficiency
polymerase chain reaction
time polymerase chain reaction
DNA
qPCR data analysis methods
amplification cycle
Actual PCR test
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