Simplified schematic diagram of the serial two-photon microscope and data acquisition process.
<p>A: The tissue is excited using a femtosecond Ti-sapphire laser (emission wavelength = 800 nm). For data collection, 50 μm of tissue (at approximately 40 μm to 90 μm below the tissue surface) is imaged in ten, 5 μm thick planes. An in-built microtome then physically removes a 50 μm thick sec...
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2021
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