DNA sequences obtained by amplifying different diatoms with different primers.

DNA sequences obtained by amplifying different diatoms with different primers.

<p>DNA sequences obtained by amplifying different diatoms with different primers.</p>

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Bibliographic Details
Main Author:	Yunchao Zhou (11978877) (author)
Other Authors:	Shan Ha (21101048) (author), Yuzhao Xu (11461289) (author), Xiaoshi Qin (21101051) (author), Yixin Ma (4595377) (author), Jianghuan Lu (21101054) (author), Bo Wang (86769) (author), Jie Cai (389349) (author), Zhiao Duan (21101057) (author), Bin Cong (313785) (author), Jianhua Chen (398446) (author), Jianqiang Deng (16931472) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Microbiology Molecular Biology Infectious Diseases Biological Sciences not elsewhere classified solving practical problems h /&# 916 40 %&# 8804 melting temperature serves neighbor method model new empirical formula resolution melting analysis dna melting temperature predicted tm values xlink "> high four specific primers corresponding gc content predicting tm value adjacent base pairs obtained dna sequences predicting tm values simple prediction method tm value obtained tm value tm values specific primers gc content base pairs melting curve method generates accurately predicting formula obtained tm position measured values hrm analysis extracted dna dna sequence following formula base composition well reflect varying levels unknown samples time monitoring thermal stability studies focused seawater diatoms sanger sequencing sample characteristics results show research subjects predictive accuracy initial stages heating process fundamental indicator fluorescence signal established system entropy change enthalpy change effectively used average error applied parameters accurately predict &# 8211
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