Confirm the outcome of the hits from 5JI4 libraries.
<p><b>A.</b> An example of peptide 5ji4-RSP-1 <i>in vitro</i> re-folding. TIC from LC/MS runs before and after re-folding are shown on the left with peaks’ m/z on the right. After two days of re-folding, both peaks 2 and 3 contain expected m/z (indicated by green triang...
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2024
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| Summary: | <p><b>A.</b> An example of peptide 5ji4-RSP-1 <i>in vitro</i> re-folding. TIC from LC/MS runs before and after re-folding are shown on the left with peaks’ m/z on the right. After two days of re-folding, both peaks 2 and 3 contain expected m/z (indicated by green triangles) for folded peptides with three disulfide bonds. <b>B.</b> An example SPR sensor gram for measuring peptide binding affinity. Kinetic fitting is on the left and steady-state affinity is on the right. Experimental data and fitting curves are shown in black and red, respectively.</p> |
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