Fast Hyperspectral and Super-Resolved Mapping of Lipid Membrane Polarity with Single-Molecule Sensitivity

Fast Hyperspectral and Super-Resolved Mapping of Lipid Membrane Polarity with Single-Molecule Sensitivity

Cell membranes display nanoscale heterogeneity in lipid composition and organization that regulates vital biological processes yet remain challenging to resolve with conventional imaging. We introduce spectral phasor single-molecule localization microscopy (SP-SMLM), a hyperspectral and super-resolu...

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Bibliographic Details
Main Author: Elric Dion Pott (22519985) (author)
Other Authors: Meek Yang (12840237) (author), James Ethan Batey (17707922) (author), Joie Embree (22519988) (author), Bin Dong (147019) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Medicine
Cell Biology
Physiology
Biotechnology
Space Science
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Physical Sciences not elsewhere classified
like optical filtering
studying membrane heterogeneity
specific polarity differences
phasor angle correlates
molecule localization microscopy
50 nm spatial
smlm revealed organelle
lipid membrane polarity
single dye molecules
membrane polarity
single molecules
simultaneous spatial
phasor space
molecule imaging
lipid composition
temporal resolutions
stained cos
spectral mean
spectral analysis
snr mapping
smlm ),
resolved mapping
resolution method
rapid acquisition
powerful tool
nile red
method ’
live cells
dynamic remodeling
conventional imaging
combines wavefront
channel imager
built three
7 cells
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