Insulin secretion assay using MIN6-6.

(A) Insulin secretion assay in MIN6-6 cells under different stimulation conditions. Cells were treated with glucose (25 mM), GLP-1 (1 µM), or both. (Glucose: ****P < 0.0001; GLP-1: **P < 0.0052; Glucose and GLP-1: ****P...

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Main Author:	Ha Neul Lee (7360352) (author)
Other Authors:	Julia C. Hardy (21781692) (author), Emily H. Pool (14883318) (author), Jin-Fan Zhang (21781695) (author), Su Hyun Kim (828853) (author), William F. Buhl (21781698) (author), Jessica G.H. Bruystens (21781701) (author), Sohum Mehta (2000764) (author), Susan S. Taylor (44167) (author), Jin Zhang (53297) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Developmental Biology Cancer Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified ubiquitous second messenger selective molecular tool reveal key roles prevent uncontrolled proliferation min6 β cells mediated gene expression facilitate spatial compartmentalization dependent protein kinase cyclic adenosine monophosphate control insulin secretion based riα knockout exhibits defective camp camp oscillation frequency div >< p 2 +</ sup studying riα llps pka ), riα camp ), riα mutant riα llps riα condensates regulatory subunit regulate creb previously showed pka type phase separation largely unknown induced llps high specificity functional impact data establish combining crispr cellular functions
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Insulin secretion assay using MIN6-6.

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