A835D reduces thermostability of the SHC014-CoV spike.
<p><b>(a)</b> scVSV-SHC014-CoV S particles were incubated at various temperatures for 1 h and used to infect DBT-9 cells overexpressing <i>Ra</i>ACE2. (average±SD, n = 4–6 from 2–3 independent experiments). Infectivity levels were normalized to the infectivity percentag...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , , , , , , |
| منشور في: |
2024
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p><b>(a)</b> scVSV-SHC014-CoV S particles were incubated at various temperatures for 1 h and used to infect DBT-9 cells overexpressing <i>Ra</i>ACE2. (average±SD, n = 4–6 from 2–3 independent experiments). Infectivity levels were normalized to the infectivity percentage at 41°C for each virus. Area under the curve (AUC) values were calculated for each curve, and groups were compared by one-way ANOVA with Dunnett’s correction for multiple comparisons. <b>(b)</b> Pre-titrated amounts of scVSV-SHC014-CoV S particles were incubated at various temperatures for 1h and loaded onto <i>Hs</i>ACE2-coated ELISA plates. A spike-specific mAb and anti-human HRP-conjugated secondary antibody were used to detect the spike protein. 9.2×10<sup>6</sup> viral GEQs per well were used. (average±SD, n = 12 from 6 independent experiments). ELISA signals were normalized to the absorbance observed for scVSV-SHC014-CoV WT at 53.5°C. Groups were compared by two-way ANOVA with Tukey’s correction for multiple comparisons, ns p>0.05; ** p<0.01; *** p<0.001; **** p<0.0001.</p> |
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