A835D reduces thermostability of the SHC014-CoV spike.
<p><b>(a)</b> scVSV-SHC014-CoV S particles were incubated at various temperatures for 1 h and used to infect DBT-9 cells overexpressing <i>Ra</i>ACE2. (average±SD, n = 4–6 from 2–3 independent experiments). Infectivity levels were normalized to the infectivity percentag...
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2024
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| _version_ | 1852025149991157760 |
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| author | Alexandra L. Tse (20235212) |
| author2 | Cory M. Acreman (20235215) Inna Ricardo-Lax (4543948) Jacob Berrigan (11052951) Gorka Lasso (215085) Toheeb Balogun (20235218) Fiona L. Kearns (11841216) Lorenzo Casalino (2850134) Georgia L. McClain (20235221) Amartya Mudry Chandran (20235224) Charlotte Lemeunier (20235227) Rommie E. Amaro (82566) Charles M. Rice (9110743) Rohit K. Jangra (6639905) Jason S. McLellan (8816639) Kartik Chandran (134142) Emily Happy Miller (10652108) |
| author2_role | author author author author author author author author author author author author author author author author |
| author_facet | Alexandra L. Tse (20235212) Cory M. Acreman (20235215) Inna Ricardo-Lax (4543948) Jacob Berrigan (11052951) Gorka Lasso (215085) Toheeb Balogun (20235218) Fiona L. Kearns (11841216) Lorenzo Casalino (2850134) Georgia L. McClain (20235221) Amartya Mudry Chandran (20235224) Charlotte Lemeunier (20235227) Rommie E. Amaro (82566) Charles M. Rice (9110743) Rohit K. Jangra (6639905) Jason S. McLellan (8816639) Kartik Chandran (134142) Emily Happy Miller (10652108) |
| author_role | author |
| dc.creator.none.fl_str_mv | Alexandra L. Tse (20235212) Cory M. Acreman (20235215) Inna Ricardo-Lax (4543948) Jacob Berrigan (11052951) Gorka Lasso (215085) Toheeb Balogun (20235218) Fiona L. Kearns (11841216) Lorenzo Casalino (2850134) Georgia L. McClain (20235221) Amartya Mudry Chandran (20235224) Charlotte Lemeunier (20235227) Rommie E. Amaro (82566) Charles M. Rice (9110743) Rohit K. Jangra (6639905) Jason S. McLellan (8816639) Kartik Chandran (134142) Emily Happy Miller (10652108) |
| dc.date.none.fl_str_mv | 2024-11-15T18:38:47Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1012704.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/A835D_reduces_thermostability_of_the_SHC014-CoV_spike_/27769604 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Neuroscience Evolutionary Biology Immunology Cancer Mental Health Infectious Diseases Virology Environmental Sciences not elsewhere classified zoonotic risks posed xlink "> understanding s2 fusion peptide cell entry mechanisms like bat cov divergent bat covs enhanced receptor binding cell entry binding domain bat covs bat coronaviruses viral entry cellular receptor enhanced spike virus bearing terminal domain successful recovery spike receptor serial passage second substitution proximal region pandemic preparedness offs inherent novel substitution fppr substitution fppr ). fitness trade coronavirus contexts bearing spikes |
| dc.title.none.fl_str_mv | A835D reduces thermostability of the SHC014-CoV spike. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(a)</b> scVSV-SHC014-CoV S particles were incubated at various temperatures for 1 h and used to infect DBT-9 cells overexpressing <i>Ra</i>ACE2. (average±SD, n = 4–6 from 2–3 independent experiments). Infectivity levels were normalized to the infectivity percentage at 41°C for each virus. Area under the curve (AUC) values were calculated for each curve, and groups were compared by one-way ANOVA with Dunnett’s correction for multiple comparisons. <b>(b)</b> Pre-titrated amounts of scVSV-SHC014-CoV S particles were incubated at various temperatures for 1h and loaded onto <i>Hs</i>ACE2-coated ELISA plates. A spike-specific mAb and anti-human HRP-conjugated secondary antibody were used to detect the spike protein. 9.2×10<sup>6</sup> viral GEQs per well were used. (average±SD, n = 12 from 6 independent experiments). ELISA signals were normalized to the absorbance observed for scVSV-SHC014-CoV WT at 53.5°C. Groups were compared by two-way ANOVA with Tukey’s correction for multiple comparisons, ns p>0.05; ** p<0.01; *** p<0.001; **** p<0.0001.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_d8f3e276fecd859f4bb0347b559c9c19 |
| identifier_str_mv | 10.1371/journal.ppat.1012704.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/27769604 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | A835D reduces thermostability of the SHC014-CoV spike.Alexandra L. Tse (20235212)Cory M. Acreman (20235215)Inna Ricardo-Lax (4543948)Jacob Berrigan (11052951)Gorka Lasso (215085)Toheeb Balogun (20235218)Fiona L. Kearns (11841216)Lorenzo Casalino (2850134)Georgia L. McClain (20235221)Amartya Mudry Chandran (20235224)Charlotte Lemeunier (20235227)Rommie E. Amaro (82566)Charles M. Rice (9110743)Rohit K. Jangra (6639905)Jason S. McLellan (8816639)Kartik Chandran (134142)Emily Happy Miller (10652108)MicrobiologyCell BiologyNeuroscienceEvolutionary BiologyImmunologyCancerMental HealthInfectious DiseasesVirologyEnvironmental Sciences not elsewhere classifiedzoonotic risks posedxlink "> understandings2 fusion peptidecell entry mechanismslike bat covdivergent bat covsenhanced receptor bindingcell entrybinding domainbat covsbat coronavirusesviral entrycellular receptorenhanced spikevirus bearingterminal domainsuccessful recoveryspike receptorserial passagesecond substitutionproximal regionpandemic preparednessoffs inherentnovel substitutionfppr substitutionfppr ).fitness tradecoronavirus contextsbearing spikes<p><b>(a)</b> scVSV-SHC014-CoV S particles were incubated at various temperatures for 1 h and used to infect DBT-9 cells overexpressing <i>Ra</i>ACE2. (average±SD, n = 4–6 from 2–3 independent experiments). Infectivity levels were normalized to the infectivity percentage at 41°C for each virus. Area under the curve (AUC) values were calculated for each curve, and groups were compared by one-way ANOVA with Dunnett’s correction for multiple comparisons. <b>(b)</b> Pre-titrated amounts of scVSV-SHC014-CoV S particles were incubated at various temperatures for 1h and loaded onto <i>Hs</i>ACE2-coated ELISA plates. A spike-specific mAb and anti-human HRP-conjugated secondary antibody were used to detect the spike protein. 9.2×10<sup>6</sup> viral GEQs per well were used. (average±SD, n = 12 from 6 independent experiments). ELISA signals were normalized to the absorbance observed for scVSV-SHC014-CoV WT at 53.5°C. Groups were compared by two-way ANOVA with Tukey’s correction for multiple comparisons, ns p>0.05; ** p<0.01; *** p<0.001; **** p<0.0001.</p>2024-11-15T18:38:47ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1012704.g005https://figshare.com/articles/figure/A835D_reduces_thermostability_of_the_SHC014-CoV_spike_/27769604CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/277696042024-11-15T18:38:47Z |
| spellingShingle | A835D reduces thermostability of the SHC014-CoV spike. Alexandra L. Tse (20235212) Microbiology Cell Biology Neuroscience Evolutionary Biology Immunology Cancer Mental Health Infectious Diseases Virology Environmental Sciences not elsewhere classified zoonotic risks posed xlink "> understanding s2 fusion peptide cell entry mechanisms like bat cov divergent bat covs enhanced receptor binding cell entry binding domain bat covs bat coronaviruses viral entry cellular receptor enhanced spike virus bearing terminal domain successful recovery spike receptor serial passage second substitution proximal region pandemic preparedness offs inherent novel substitution fppr substitution fppr ). fitness trade coronavirus contexts bearing spikes |
| status_str | publishedVersion |
| title | A835D reduces thermostability of the SHC014-CoV spike. |
| title_full | A835D reduces thermostability of the SHC014-CoV spike. |
| title_fullStr | A835D reduces thermostability of the SHC014-CoV spike. |
| title_full_unstemmed | A835D reduces thermostability of the SHC014-CoV spike. |
| title_short | A835D reduces thermostability of the SHC014-CoV spike. |
| title_sort | A835D reduces thermostability of the SHC014-CoV spike. |
| topic | Microbiology Cell Biology Neuroscience Evolutionary Biology Immunology Cancer Mental Health Infectious Diseases Virology Environmental Sciences not elsewhere classified zoonotic risks posed xlink "> understanding s2 fusion peptide cell entry mechanisms like bat cov divergent bat covs enhanced receptor binding cell entry binding domain bat covs bat coronaviruses viral entry cellular receptor enhanced spike virus bearing terminal domain successful recovery spike receptor serial passage second substitution proximal region pandemic preparedness offs inherent novel substitution fppr substitution fppr ). fitness trade coronavirus contexts bearing spikes |