Representative standard curve for conversion of Cq values to DNA quantity.

Representative standard curve for conversion of Cq values to DNA quantity.

<p>Representative calibration curve illustrating qPCR standards across a range of concentrations from 0.01–1000 pg/μL (0.05–5000 pg/rxn) of purified MTB DNA (mean ± SD, n = 7).</p> <p>(TIF)</p>

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Bibliographic Details
Main Author:	Alexander J. Yan (16618734) (author)
Other Authors:	Alaina M. Olson (10810548) (author), Kris M. Weigel (9585310) (author), Angelique K. Luabeya (16949162) (author), Erin Heiniger (9362518) (author), Mark Hatherill (23097) (author), Gerard A. Cangelosi (165934) (author), Paul Yager (366005) (author)
Published:	2024
Subjects:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Biotechnology Cancer Infectious Diseases Space Science Biological Sciences not elsewhere classified Mathematical Sciences not elsewhere classified Chemical Sciences not elsewhere classified varied diagnostic performance target nucleic acid spiked tongue swabs pcr assays compared may inhibit pcr div >< p sample preparation method mycobacterium tuberculosis </ specificity may also demonstrated better specificity mtb dna yield specific hybridization capture show improved sensitivity qiagen dna extraction previously reported methods sputum xpert mtb hybridization capture invasive sample diagnosing tuberculosis mtb lysis tb ). study conducted south africa rif ultra reference standard magnetic beads incomplete lysis fold increase ethanol precipitation combines sonication clinical samples care platforms
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