A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase
<i>N</i>-acetyl-β-<i>d</i>-glucosaminidase (NAG) is an important biomarker that indicates early renal tubular damage. Its activity level in urine has been widely used for early detection and monitoring of chronic kidney disease (CKD), particularly diabetic nephropathy. Howeve...
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2025
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| _version_ | 1849927640250580992 |
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| author | Jie Cheng (294193) |
| author2 | Taiqiang Zhao (22677110) Jiasheng Yan (10287043) Zhengkang Chu (22677113) Yongmei Tian (560990) Shi Hua (20154516) Meiling Jin (3975752) Peihong Xiao (1395568) Jinhong Guo (613139) Honghua Hu (1272258) |
| author2_role | author author author author author author author author author |
| author_facet | Jie Cheng (294193) Taiqiang Zhao (22677110) Jiasheng Yan (10287043) Zhengkang Chu (22677113) Yongmei Tian (560990) Shi Hua (20154516) Meiling Jin (3975752) Peihong Xiao (1395568) Jinhong Guo (613139) Honghua Hu (1272258) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jie Cheng (294193) Taiqiang Zhao (22677110) Jiasheng Yan (10287043) Zhengkang Chu (22677113) Yongmei Tian (560990) Shi Hua (20154516) Meiling Jin (3975752) Peihong Xiao (1395568) Jinhong Guo (613139) Honghua Hu (1272258) |
| dc.date.none.fl_str_mv | 2025-11-24T22:14:03Z |
| dc.identifier.none.fl_str_mv | 10.1021/acssensors.5c02754.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/media/A_Sequential_Dual-Reagent_Paper-Based_Analytical_Device_with_Structural_Release_Control_for_Point-of-Care_Detection_of_Urinary_i_N_i_Acetyl-_i_d_i_Glucosaminidase/30698765 |
| dc.rights.none.fl_str_mv | CC BY-NC 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Cell Biology Genetics Biotechnology Developmental Biology Marine Biology Space Science Biological Sciences not elsewhere classified Information Systems not elsewhere classified utilizes structural partitioning system uses vra optical quantitative analysis initiate color development color development process structural release control based analytical device linear detection range standard clinical method nag enzymatic reaction chronic kidney disease 2 </ sup kidney disease programmed control portable device method provides >< sup early detection detection limit care detection β ‑< β -< widely used trigger mechanism strong potential sequential dual requires portability reagent paper r </ programmed delay n </ important biomarker high risk high reproducibility findings highlight correlation coefficient complex instrumentation clinical poct care testing alkaline catalyst activity level achieve time >‑ glucosaminidase >‑ acetyl >- glucosaminidase >- acetyl 6 %, 524 u 5 ). |
| dc.title.none.fl_str_mv | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| dc.type.none.fl_str_mv | Dataset Media info:eu-repo/semantics/publishedVersion dataset |
| description | <i>N</i>-acetyl-β-<i>d</i>-glucosaminidase (NAG) is an important biomarker that indicates early renal tubular damage. Its activity level in urine has been widely used for early detection and monitoring of chronic kidney disease (CKD), particularly diabetic nephropathy. However, current detection methods rely on complex instrumentation, which hinders their suitability for point-of-care testing (POCT) that requires portability and user-friendliness. To address this challenge, we developed a sequential dual-reagent paper-based analytical device (SeDR-PAD) that utilizes structural partitioning and a delayed-trigger mechanism to achieve time-programmed control of the NAG enzymatic reaction and color development process. The system uses VRA-GlcNAc as the substrate to generate a chromogen under the catalysis of NAG. A portable device then releases an alkaline catalyst after a programmed delay to initiate color development, followed by optical quantitative analysis. This method provides a linear detection range of 0–200 U/L, with a detection limit of 0.524 U/L and high reproducibility (CV < 6%, <i>n</i> = 5). In a methodological comparison involving 70 clinical urine samples, the SeDR-PAD platform demonstrated strong concordance with the standard clinical method, with a correlation coefficient <i>R</i><sup>2</sup> of 0.9833. These findings highlight the strong potential of SeDR-PAD for clinical POCT of uNAG in individuals at high risk for kidney disease. |
| eu_rights_str_mv | openAccess |
| id | Manara_db50dc3a85b6bc4385595bffba04b59a |
| identifier_str_mv | 10.1021/acssensors.5c02754.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30698765 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY-NC 4.0 |
| spelling | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑GlucosaminidaseJie Cheng (294193)Taiqiang Zhao (22677110)Jiasheng Yan (10287043)Zhengkang Chu (22677113)Yongmei Tian (560990)Shi Hua (20154516)Meiling Jin (3975752)Peihong Xiao (1395568)Jinhong Guo (613139)Honghua Hu (1272258)MedicineCell BiologyGeneticsBiotechnologyDevelopmental BiologyMarine BiologySpace ScienceBiological Sciences not elsewhere classifiedInformation Systems not elsewhere classifiedutilizes structural partitioningsystem uses vraoptical quantitative analysisinitiate color developmentcolor development processstructural release controlbased analytical devicelinear detection rangestandard clinical methodnag enzymatic reactionchronic kidney disease2 </ supkidney diseaseprogrammed controlportable devicemethod provides>< supearly detectiondetection limitcare detectionβ ‑<β -<widely usedtrigger mechanismstrong potentialsequential dualrequires portabilityreagent paperr </programmed delayn </important biomarkerhigh riskhigh reproducibilityfindings highlightcorrelation coefficientcomplex instrumentationclinical poctcare testingalkaline catalystactivity levelachieve time>‑ glucosaminidase>‑ acetyl>- glucosaminidase>- acetyl6 %,524 u5 ).<i>N</i>-acetyl-β-<i>d</i>-glucosaminidase (NAG) is an important biomarker that indicates early renal tubular damage. Its activity level in urine has been widely used for early detection and monitoring of chronic kidney disease (CKD), particularly diabetic nephropathy. However, current detection methods rely on complex instrumentation, which hinders their suitability for point-of-care testing (POCT) that requires portability and user-friendliness. To address this challenge, we developed a sequential dual-reagent paper-based analytical device (SeDR-PAD) that utilizes structural partitioning and a delayed-trigger mechanism to achieve time-programmed control of the NAG enzymatic reaction and color development process. The system uses VRA-GlcNAc as the substrate to generate a chromogen under the catalysis of NAG. A portable device then releases an alkaline catalyst after a programmed delay to initiate color development, followed by optical quantitative analysis. This method provides a linear detection range of 0–200 U/L, with a detection limit of 0.524 U/L and high reproducibility (CV < 6%, <i>n</i> = 5). In a methodological comparison involving 70 clinical urine samples, the SeDR-PAD platform demonstrated strong concordance with the standard clinical method, with a correlation coefficient <i>R</i><sup>2</sup> of 0.9833. These findings highlight the strong potential of SeDR-PAD for clinical POCT of uNAG in individuals at high risk for kidney disease.2025-11-24T22:14:03ZDatasetMediainfo:eu-repo/semantics/publishedVersiondataset10.1021/acssensors.5c02754.s002https://figshare.com/articles/media/A_Sequential_Dual-Reagent_Paper-Based_Analytical_Device_with_Structural_Release_Control_for_Point-of-Care_Detection_of_Urinary_i_N_i_Acetyl-_i_d_i_Glucosaminidase/30698765CC BY-NC 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306987652025-11-24T22:14:03Z |
| spellingShingle | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase Jie Cheng (294193) Medicine Cell Biology Genetics Biotechnology Developmental Biology Marine Biology Space Science Biological Sciences not elsewhere classified Information Systems not elsewhere classified utilizes structural partitioning system uses vra optical quantitative analysis initiate color development color development process structural release control based analytical device linear detection range standard clinical method nag enzymatic reaction chronic kidney disease 2 </ sup kidney disease programmed control portable device method provides >< sup early detection detection limit care detection β ‑< β -< widely used trigger mechanism strong potential sequential dual requires portability reagent paper r </ programmed delay n </ important biomarker high risk high reproducibility findings highlight correlation coefficient complex instrumentation clinical poct care testing alkaline catalyst activity level achieve time >‑ glucosaminidase >‑ acetyl >- glucosaminidase >- acetyl 6 %, 524 u 5 ). |
| status_str | publishedVersion |
| title | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| title_full | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| title_fullStr | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| title_full_unstemmed | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| title_short | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| title_sort | A Sequential Dual-Reagent Paper-Based Analytical Device with Structural Release Control for Point-of-Care Detection of Urinary <i>N</i>‑Acetyl-β‑<i>d</i>‑Glucosaminidase |
| topic | Medicine Cell Biology Genetics Biotechnology Developmental Biology Marine Biology Space Science Biological Sciences not elsewhere classified Information Systems not elsewhere classified utilizes structural partitioning system uses vra optical quantitative analysis initiate color development color development process structural release control based analytical device linear detection range standard clinical method nag enzymatic reaction chronic kidney disease 2 </ sup kidney disease programmed control portable device method provides >< sup early detection detection limit care detection β ‑< β -< widely used trigger mechanism strong potential sequential dual requires portability reagent paper r </ programmed delay n </ important biomarker high risk high reproducibility findings highlight correlation coefficient complex instrumentation clinical poct care testing alkaline catalyst activity level achieve time >‑ glucosaminidase >‑ acetyl >- glucosaminidase >- acetyl 6 %, 524 u 5 ). |