Average cycle threshold values following qPCR amplification with FaRu primers.

<p>(A) Sample DNA was extracted with DNeasy and TaqMan chemistry was used, (B) PowerWater TaqMan, (C) DNeasy SYBR, (D) PowerWater SYBR. Cutoff values set at 34 for TaqMan chemistry (A) and (B) and 32 for SYBR (C) and (D). Site codes as in <a href="http://www.plosone.org/article/info:do...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Stephanie S. Coster (3293484) (author)
مؤلفون آخرون:	Megan N. Dillon (11624726) (author), William Moore (2403961) (author), George T. Merovich Jr. (11624729) (author)
منشور في:	2021
الموضوعات:	Biophysics Biochemistry Genetics Molecular Biology Biotechnology Ecology Cancer Inorganic Chemistry Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified sybr green ). proper sample processing powerwater kits ), monitor aquatic systems juniata river watershed div >< p becoming increasingly popular species specificity compared mitochondrial dna shed invasive rusty crayfish faxonius rusticus </ taqman qpcr chemistry invasive species rusticus </ stream crayfish f </ tissue kit qiagen ’ performed optimally noninvasive way noninvasive detection maximize sensitivity longmire ’ filter samples environmental dna central pennsylvania assays based
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Average cycle threshold values following qPCR amplification with FaRu primers.

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