Average cycle threshold values following qPCR amplification with FaRu primers.

Average cycle threshold values following qPCR amplification with FaRu primers.

<p>(A) Sample DNA was extracted with DNeasy and TaqMan chemistry was used, (B) PowerWater TaqMan, (C) DNeasy SYBR, (D) PowerWater SYBR. Cutoff values set at 34 for TaqMan chemistry (A) and (B) and 32 for SYBR (C) and (D). Site codes as in <a href="http://www.plosone.org/article/info:do...

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Bibliographic Details
Main Author: Stephanie S. Coster (3293484) (author)
Other Authors: Megan N. Dillon (11624726) (author), William Moore (2403961) (author), George T. Merovich Jr. (11624729) (author)
Published: 2021
Subjects:
Biophysics
Biochemistry
Genetics
Molecular Biology
Biotechnology
Ecology
Cancer
Inorganic Chemistry
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
sybr green ).
proper sample processing
powerwater kits ),
monitor aquatic systems
juniata river watershed
div >< p
becoming increasingly popular
species specificity compared
mitochondrial dna shed
invasive rusty crayfish
faxonius rusticus </
taqman qpcr chemistry
invasive species
rusticus </
stream crayfish
f </
tissue kit
qiagen ’
performed optimally
noninvasive way
noninvasive detection
maximize sensitivity
longmire ’
filter samples
environmental dna
central pennsylvania
assays based
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Summary:<p>(A) Sample DNA was extracted with DNeasy and TaqMan chemistry was used, (B) PowerWater TaqMan, (C) DNeasy SYBR, (D) PowerWater SYBR. Cutoff values set at 34 for TaqMan chemistry (A) and (B) and 32 for SYBR (C) and (D). Site codes as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0259084#pone.0259084.t001" target="_blank">Table 1</a>. Presence is noted as A for putative absence, P for presence, B for both <i>F</i>. <i>rusticus</i> and <i>F</i>. <i>obscurus</i>, and U for unknown.</p>

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